OCCURRENCE AND MOLECULAR DETECTION OF CAMPYLOBACTER JEJUNI AND COLI IN TABLE EGGS AND STOOL OF DIARRHEIC PATIENTS | ||||
| Assiut Veterinary Medical Journal | ||||
| Article 20, Volume 71, Issue 185, April 2025, Page 276-288 PDF (546.23 K) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/avmj.2025.354429.1559 | ||||
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| Authors | ||||
MARWA G. ABD EL KADER 1; REEM M. ALSAADAWY  2; ASMAA A. RAYAN 3
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| 1Department of Food Hygiene, Safety and Technology, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt. | ||||
| 2Department of Zoonoses, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt. | ||||
| 3Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Assiut University, 71526, Assiut Egypt. | ||||
| Abstract | ||||
| Inappropriate handling of eggs can still result in infection from bacteria such as Campylobacter, which mostly contaminates the surface of the eggshell, so this study focused on the molecular detection of Campylobacter jejuni and Campylobacter coli in table eggs and stool of diarrheic patients. The conventional PCR was carried out on 40 egg samples, 20 from each farm and baladi types equally, and 20 stool samples from diarrheic patients. It was found that Campylobacter spp. infection was prevalent in farm and baladi eggshell samples, and positive C. jejuni DNA was detected in 45% and 15%, respectively. In comparison, 50% and 40% of farm and baladi egg shell samples were positive C. coli. However, neither C. jejuni nor C. coli DNA was detected in the egg contents of either farm or baladi eggs. The prevalence of Campylobacter spp. infection was 70% and 50% in farm and baladi eggshell samples, respectively. C. coli was detected in 50% of diarrheic patients' stool, while C. jejuni wasn't detected. The prevalence of C. coli infection was higher in females than males. The 3Y-5Y age group had the highest prevalence (75%) and the lowest (40%) was in the more than 5Y group. The prevalence of C. coli infection in diarrheic patients was highest in patients with vomiting, followed by dehydration, abdominal pain, and fever. The study recommended good hygienic practices inside poultry farms and farmer's houses and increasing awareness of poultry farm workers and farmers from infection. | ||||
| Keywords | ||||
| Eggs; stool; C. jejuni; C. coli; PCR | ||||
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