MITIGATION OF HEAT STRESS EFFECT ON IN VITRO MATURED OOCYTES USING VITAMIN C IN GOAT | ||||
| Egyptian Journal of Animal Production | ||||
| Article 6, Volume 62, Issue 1, January 2025, Page 55-60 PDF (481.67 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejap.2025.354537.1101 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Hesham Shedeed 
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| Animal and Poultry Production Division, Desert Research Center, Cairo 11753, Egypt. | ||||
| Abstract | ||||
| The current investigation aimed to assess the effect of induced heat stress (40 and 42 ºC) on the maturation of goat oocyte in vitro. In addition to study the effect of vitamin C supplementation during IVM as a way to mitigate the heat stress effects. A total of 438 of oocyteswere recovered from goat ovaries using slicing technique. The oocytes were cultured in prewarmed (38.5, 40, and 42 ºC, according to the experimental group) IVM medium with or without vitamin C (VC), under mineral oil in CO2 incubator 5% CO2, with 95% humidity. Six treatment groups were established: T1 (control, 38.5°C, no vitamin C), T2 (38.5°C, 50 µg/mL vitamin C), T3 (40°C, 50 µg/mL vitamin C), T4 (40°C, no vitamin C), T5 (42°C, 50 µg/mL vitamin C), and T6 (42°C, no vitamin C). The results of cumulus expansion that were obtained in this study were as follows; 81.67, 90, 87.22, 72.67, 71.69, and 55.59% for T1, T2, T3, T4, T5, and T6 groups, respectively. The data of the first polar body extrusion were recorded as 25.66, 41.33, 31.11, 17.56, 25.86, and 10.55% for T1, T2, T3, T4, T5, and T6 groups, respectively. In conclusion, heat stress (42 ºC) significantly reduced goat oocyte’s maturation in vitro, however, vitamin C supplementation (50 µg/mL) to IVM medium may improveoocyte maturation rate and/or reduced the negative effects of heat stress. | ||||
| Keywords | ||||
| Goat; oocyte; IVM; heat-stress; vitamin C | ||||
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