Efficacy of rapid diagnostic tests for trichomoniasis and bacterial vaginitis | ||||
| Parasitologists United Journal | ||||
| Article 7, Volume 18, Issue 1, April 2025, Page 58-65 PDF (484.07 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/puj.2025.355302.1283 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Waleed M Tawfik1; Heba M Aboelela  2; Hasnaa S Abdelhamid3; Abdelbar M Sharaf4
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| 1Department of Obstetrics & Gynecology, Faculty of Medicine, Benha University, Benha, Egypt. | ||||
| 2Department of Parasitology, Faculty of Medicine, Benha University, Benha, Egypt. | ||||
| 3Department of Medical Microbiology and Immunology, Faculty of Medicine, Benha University, Benha, Egypt. | ||||
| 4Department of Obstetrics & Gynecology, Faculty of Medicine, Menoufia University, Menoufia, Egypt. | ||||
| Abstract | ||||
| Background: Mixed Trichomonas vaginitis (TV) and bacterial vaginosis (BV) seriously affect females’ reproductive and sexual health. Misdiagnosis may predispose to continued symptoms and inappropriate treatment. This necessitated trials to evaluate the diagnostic performance of simple tests to rapidly and accurately diagnose vulvovaginitis (VV). Objective: To evaluate the efficacy of OSOM® Trichomonas rapid test and the comprehensive 4-parameter (C-4-P) test for TV and BV, respectively in women with VV. Patients and Methods: Vaginal swabs of 85 non-pregnant women with VV were collected. Diagnosis of TV was achieved by anaerobic broth culturing of swabs, the capillary-flow dipstick OSOM® rapid test for detection of antigen, the colorimetric detection of the sialidase enzyme activity (SNA) and leukocyte esterase (LE), and determination of vaginal pH and H2O2 levels by the C-4-P test. Diagnosis of BV was by the Nugent scoring system for Gram-stained vaginal smear. Results: The culture detected 8 positive specimens for trichomoniasis (9.4%). The OSOM® test detected positive cases with 100% specificity and positive predictive value (PPV), with 87.5% sensitivity and 98.72% negative predictive value (NPV), and 98.82% accuracy rate. The Nugent scoring system defined 25 negative, 39 intermediate, and 21 positive smears for BV. The C-4-P test differentiated the intermediate smears as 27 positives and 12 negatives and showed sensitivity and specificity rates of 95.24% and 92%, respectively with PPV and NPV of 90.91 and 93.48 and 93.48% accuracy rate for BV diagnosis. Conclusion: The OSOM® and the C-4-P rapid tests | ||||
| Keywords | ||||
| bacterial vaginosis; C-4-P test; nugent scoring system; OSOM® rapid test; trichomoniasis | ||||
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