MiR-30a as a Noninvasive Diagnostic and Prognostic Biomarker in Pediatric Nephrotic Syndrome
Mohareb, D., Mohamed, H., Hafez, R., Hassan, S., Taha, S., Abdel Rahman, E. (2025). MiR-30a as a Noninvasive Diagnostic and Prognostic Biomarker in Pediatric Nephrotic Syndrome. EKB Journal Management System, 48(1), 457-468. doi: 10.21608/bfsa.2025.351497.2398
Dina Ahmed Mohareb; Hanan omar Mohamed; Rania Azmy Hafez; Samaher Fathy Hassan; Sherin A Taha; Eman Mohamed Abdel Rahman. "MiR-30a as a Noninvasive Diagnostic and Prognostic Biomarker in Pediatric Nephrotic Syndrome". EKB Journal Management System, 48, 1, 2025, 457-468. doi: 10.21608/bfsa.2025.351497.2398
Mohareb, D., Mohamed, H., Hafez, R., Hassan, S., Taha, S., Abdel Rahman, E. (2025). 'MiR-30a as a Noninvasive Diagnostic and Prognostic Biomarker in Pediatric Nephrotic Syndrome', EKB Journal Management System, 48(1), pp. 457-468. doi: 10.21608/bfsa.2025.351497.2398
Mohareb, D., Mohamed, H., Hafez, R., Hassan, S., Taha, S., Abdel Rahman, E. MiR-30a as a Noninvasive Diagnostic and Prognostic Biomarker in Pediatric Nephrotic Syndrome. EKB Journal Management System, 2025; 48(1): 457-468. doi: 10.21608/bfsa.2025.351497.2398

MiR-30a as a Noninvasive Diagnostic and Prognostic Biomarker in Pediatric Nephrotic Syndrome

Bulletin of Pharmaceutical Sciences Assiut University
Volume 48, Issue 1, June 2025, Page 457-468  XML PDF (1018.24 K)
Document Type: Original Article
DOI: 10.21608/bfsa.2025.351497.2398
View on SCiNiTO View on SCiNiTO
Authors
Dina Ahmed Mohareb1; Hanan omar Mohamed1; Rania Azmy Hafez email 1; Samaher Fathy Hassan2; Sherin A Taha3; Eman Mohamed Abdel Rahman1
1Clinical Pathology Department, Assiut University, Faculty of Medicine
2Pediatric Department, Faculty of Medicine, Assiut university
3Pediatric Department, Faculty of Medicine, Seuz University
Abstract
Nephrotic syndrome (NS), a common pediatric kidney disorder, is characterized by severe proteinuria, leading to hypoalbuminemia, hyperlipidemia, edema, and various life-threatening complications. Early detection is critical for reducing disease progression and mitigating associated risks, highlighting the need to identify noninvasive biomarkers that can improve diagnostic accuracy. MicroRNAs (miRNAs), particularly miR-30a, have emerged as promising biomarkers for kidney diseases due to their involvement in key pathological processes such as inflammation and fibrosis. This study aimed to assess the diagnostic and prognostic utility of miR-30a in pediatric NS by evaluating its expression across different clinical subtypes. This study was conducted on 100 children from those enrolled at Assiut University Hospital. Participants were divided into five groups: Group 1 (Control), Group 2 (Steroid Sensitive), Group 3 (Steroid Resistant), Group 4 (Immunosuppressive Resistant), and Group 5 (Refractory NS), with 20 participants in each group. The expression level of miR-30a was quantified using quantitative real-time PCR (qRT-PCR). The results demonstrated significantly elevated miR-30a levels in NS patients compared to controls. Receiver operating characteristic (ROC) curve analysis demonstrated a high diagnostic accuracy of miR-30a in distinguishing NS patients from healthy controls, with an AUC of 0.920 (95% CI: 0.894–0.946; P < 0.001), an accuracy of 94.5%, a sensitivity of 88.6%, and a specificity of 100.0%. It also showed strong discriminatory power among the various NS subtypes. These findings suggest that miR-30a may serve as a promising diagnostic and prognostic biomarker, providing valuable insights for treatment stratification in pediatric NS.
Keywords
Keywords: Nephrotic Syndrome; Pediatric kidney disorder; Proteinuria; MicroRNAs; miR-30a
Statistics
Article View: 221
PDF Download: 54