Antimicrobial and Antibiofilm Potentials of Methanolic Extract from Cultivated Tropical Hibiscus (Hibiscus rosa sinensis L.) Flower | ||||
| Egyptian Academic Journal of Biological Sciences, G. Microbiology | ||||
| Volume 17, Issue 1, June 2025, Page 109-120 PDF (898.48 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/eajbsg.2025.423484 | ||||
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| Authors | ||||
Noura Sh. A. Hagaggi; Usama M. Abdul- Raouf; Doaa M. A. Khalil
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| Botany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt. | ||||
| Abstract | ||||
| Using natural antimicrobials to treat microbial infections is one of the hopeful alternatives to combating antibiotic-resistant strains throughout the world. In this work, the antimicrobial and antibiofilm properties of methanolic extract derived from tropical hibiscus flower were investigated against a variety of microbial pathogens. Results showed that the extract contained considerable amounts of anthocyanins, polyphenols, and flavonoids. Furthermore, the extract contained high amounts of volatile compounds: Disulfide, di-tert-dodecyl (11.378%), Tridecanol, 2-ethyl-2-methyl- (11.940%), Didodecyl phthalate (13.701%), Eicosane (14.532%), and Octane, 2-methyl- (15.242%). Interestingly, the growth, cell morphology, and biofilms of the tested pathogenic bacteria were highly affected by the extract. High sensitivity towards the extract was exhibited by the tested bacteria: Staphylococcus aureus (36±0.1 mm), Micrococcus luteus (45±0.5 mm), Escherichia coli (45±0.15 mm), and Klebsiella pneumonia (40±0.5 mm), with MIC values of 1, 0.5, 0.5 and 1 mg/mL, respectively. The extract inhibited the formation of biofilm in Staphylococcus aureus, Micrococcus luteus, Escherichia coli and Klebsiella pneumonia by 46.4, 47.6, 46.3 and 38.4% respectively. The strongest antifungal activity of the extract was against Fusarium solani (34.7%) and Alternaria phragmospora (33.8%) at 8 mg/mL. The findings of this study indicated that tropical hibiscus flower extract may be promising for the development and design of natural antimicrobial alternatives. | ||||
| Keywords | ||||
| Antimicrobial; Antibiofilm; Extract; Staphylococcus aureus; Hibiscus | ||||
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