Effect of Certain Biocontrol Agents on The Root-Knot Nematode and Some Enzymatic Activities in Tomato Rhizosphere | ||
| Alexandria Journal of Agricultural Sciences | ||
| Article 1, Volume 70, Issue 3, September 2025, Pages 236-246 PDF (464.22 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/alexja.2025.373299.1136 | ||
| Authors | ||
| Alaa H. A. Abu Habib1; Hala H. Badry2; Abeer Mohamed* 1; Manal M. Zen El-Dein3 | ||
| 1Plant Pathology Research Institute, Agricultural Research Center (ARC), Alexandria 21616, Egypt | ||
| 2Soil and Water Science Department, Faculty of Agriculture, Alexandria University, Aflaton Street, El-Shatby, EG21545 Alexandria, Egypt | ||
| 3Fungicide, Bactericide and Nematicide Research Department, Central Agricultural Pesticide Laboratory (CAPL), Agriculture Research Center (ARC), Alexandria 21616, Egypt | ||
| Abstract | ||
| Root-knot nematodes (Meloidogyne spp.) are a group of the most destructive plant pathogens of tomato plants all over the world. Biological control is considered as a safe and efficient tool to control these pathogens. The present study aimed to evaluate the potency of the marine algae (Ulva fasciata) and two bacterial isolates isolated from the seeds and rhizosphere of tomato plants against the root-knot nematode, M. incognita,infecting tomato plants. The bacterial isolates were identified using polymerase chain reaction (PCR) through the amplification of 16S rRNA as Bacillus amyloliquefaciens (PQ821314) and Serratia marcescens (PQ012666). Bacillus amyloliquefaciens and S. marcescens were used at 108 CFU/ml, while U. fasciata was used at 5000, 2500, 1250 and 625 mg/l concentrations in vitro. A comparable treatments included; Nemacross® 2% and oxamyl 24% (SL) beside the nontreated check. Results showed that B. amyloliquefaciens, S. marcescens, U. fasciata and oxamyl significantly reduced (P ≤ 0.05) egg hatching of M. incognita and increased (P ≤ 0.05) the mortality of M. incognita J2s, compared to the control checks. However, Nemacross® caused 28.84% larval mortality and 23.72% egg hatchability. Under greenhouse conditions, the disease severity (root galling) and the nematode reproduction (no. egg mass/plant) of M. incognita were greatly suppressed by all treatments. Maximum reduction of root galling (98.77%) and nematode reproduction (98.10%) was obtained by B. amyloliquefaciens. Fresh weights of shoot and root systems were generally increased by the application of the nematicide oxamyl and all the tested bioagents, compared to the control treatment (M. incognita). The soil enzyme activities were also evaluated under greenhouse conditions. The application of the biological products in nematode-infested soils significantly (P ≤ 0.05) enhanced the soil enzyme activities, particularly urease, dehydrogenase, and alkaline phosphatase. However, oxamyl showed detrimental effects on soil enzyme activities. | ||
| Keywords | ||
| Alkaline phosphatase; Bacillus amyloliquefaciens; dehydrogenase, Meloidogyne incognita; Serratia marcescens | ||
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