Enhancing Growth, Immunity and Disease Resistance in Nile Tilapia (Oreochromis niloticus) Through Dietary Sodium Butyrate Supplementation

Moustafa, Eman Moustafa; Shukry, Mustafa; Assas, Mona; Khallaf, Mohamed A.; Ghetas, Hanan A.; hafez, azza Ahmed; Marzouk, Wesam H.

doi:10.21608/ejvs.2025.368505.2701

	Enhancing Growth, Immunity and Disease Resistance in Nile Tilapia (Oreochromis niloticus) Through Dietary Sodium Butyrate Supplementation
Egyptian Journal of Veterinary Sciences
Articles in Press, Corrected Proof, Available Online from 23 April 2025 PDF (1.27 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2025.368505.2701
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Authors
Eman Moustafa Moustafa ¹; Mustafa Shukry ²; Mona Assas ³; Mohamed A. Khallaf ⁴; Hanan A. Ghetas ⁴; azza Ahmed hafez ⁵; Wesam H. Marzouk ¹
¹Egypt, Kafr El-Sheikh University, Faculty of Veterinary Medicine, Fish Diseases and Management Department
²Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
³Fish processing and biotechnology department, Faculty of Aquatic and fisheries sciences, Kafrelsheikh University, Kafrelsheikh 33516, Egypt
⁴Department of Aquatic Animals Medicine and Management, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Egypt.
⁵kafrelsheikh
Abstract
This study investigates the effects of dietary sodium butyrate supplementation (0.5% and 1%) on the growth performance, hematological and biochemical parameters, oxidative stress markers, and gene expression of Nile tilapia (Oreochromis niloticus) before and after exposure to Aeromonas hydrophila. Fish were fed sodium butyrate-supplemented diets for 56 days, after which they were challenged with A. hydrophila to evaluate immune responses and disease resistance. Growth parameters, including weight gain and feed conversion ratio, significantly improved in sodium butyrate-fed fish, with the 0.5% group exhibiting the highest growth performance. Hematological indices such as red blood cell count, hemoglobin, and packed cell volume increased in supplemented groups, indicating improved oxygen transport and immune readiness. Biochemical markers showed reduced ALT and AST levels, suggesting enhanced liver function, while higher total protein and globulin levels reflected improved immune competence. Sodium butyrate also enhanced antioxidant defense mechanisms; evidenced by increased superoxide dismutase, catalase, and glutathione peroxidase activities and decreased malondialdehyde levels. Gene expression analysis revealed an upregulation of TNF-α and IL-1β before infection, indicating a pre-activated immune system without excessive inflammation. At the same time, post-infection, sodium butyrate-fed fish exhibited higher NF-κB, TNF-α, and IL-1β expression, suggesting a stronger immune response. After infection, sodium butyrate-supplemented fish maintained better hematological and biochemical profiles, experienced lower oxidative stress, and exhibited enhanced immune responses compared to the control group. The findings suggest that dietary sodium butyrate, particularly at 0.5%, can significantly improve Nile tilapia's growth, immunity, and disease resistance, making it a promising functional feed additive for sustainable aquaculture.
Keywords
Sodium butyrate; Nile tilapia; Aeromonas hydrophila; immune response; disease resistance


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