IN VITRO ANTIMICROBIAL PHOTODYNAMIC ACTIVITY OF TECOMA STANS YELLOW FLOWERS EXTRACT LOADED IN NANO-EMULSION | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Volume 48, Issue 1, June 2025, Pages 175-187 PDF (1.16 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2025.365135.2465 | ||
| Authors | ||
| Asmaa khattab1; Nagwa E Awad2; Doaa Abdel Faddel* 3; Maha Fadel4 | ||
| 1Department of Medical Applications of Laser, National Institute of Laser Enhanced Sciences (NILES), Cairo University, Egypt | ||
| 2Pharmacognosy Department, National Research Centre, Dokki, Giza, Egypt | ||
| 3Department of medical applications of laser, National Institute of Laser Enhanced Sciences, Cairo University | ||
| 4Department of medical laser applications, The national Institute of Laser Enhanced Sciences, cairo university. | ||
| Abstract | ||
| Antimicrobial photodynamic therapy (aPDT) is an approach that was recently used to combat the resistance developed by pathogens toward antimicrobial drugs. Tecoma stans (L.) Jus., a prevalent tree in Egypt, was reported to be a promising photosensitizer in PDT. This study investigates the antimicrobial photodynamic activity of the methanolic crude extract of Tecoma stans (L.) Juss. ex Kunth (T. stans) loaded in a nano-emulsion as a natural photosensitizer. Molecular docking and molecular dynamics studies were conducted to investigate the binding between delphinidin as a promising lead and DNA's active site and to assess its stability through simulation The nano-emulsion formulation (TSFE-NE) was prepared, characterized, and evaluated for its antimicrobial photodynamic activities against three types of microorganisms: Gram-negative bacteria (Escherichia coli), Gram-positive bacteria (Staphylococcus aureus), and the pathogenic yeast Candida albicans. The percentage of microbial eradication was calculated after irradiation at 90 Mw/cm2. The microbial eradication of TSFE-NE was enhanced after light exposure. Eradication percentages of 97.43%, 90.56%, and 87.77% were obtained for Escherichia coli, Staphylococcus aureus, and Candida albicans, respectively. These results confirmed the efficacy of natural photosensitizer and nanotechnology combination for improving antimicrobial photodynamic activities | ||
| Keywords | ||
| Tecoma stans flower extract; nano-emulsion; antimicrobial photodynamic therapy; natural photosensitizers; molecular dynamics | ||
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