Culture Conditions Influencing Growth and Antibiotic Synthesis by Streptomyces sp. strain MFB28 | ||
| Bulletin of Pharmaceutical Sciences Assiut University | ||
| Articles in Press, Accepted Manuscript, Available Online from 28 April 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bfsa.2025.352605.2403 | ||
| Authors | ||
| Nadia Zougagh* ; Zineb Azoubi; Assia Mouslim; Ayoub El Abbassi; Mohammed El Khasmi; Mohammed Menggad | ||
| Laboratory of Ecology and Environment, Department of Biology, Faculty of Sciences Ben M’sick, University Hassan II of Casablanca, Sidi Othman, BP 7955, Casablanca, Morocco | ||
| Abstract | ||
| A soil isolated Streptomyces sp. strain MFB28 is a promising Gram-positive antibacterial compounds producer. The optimization of secondary metabolite production was explored by assessing factors such as Culture media, incubation time, inoculum size, pH levels, temperature (°C) and phosphorus concentration. Secondary metabolites released in culture medium were extracted in chloroform and the remaining were kept in the aqueous medium solution and extracted from the mycelium with ethanol. Their antibacterial activity was tested against three Gram-positive bacteria via disk diffusion. All extracts showed antibacterial activity. Optimal biomass and bioactive metabolite production occurred on the 5th day, using an inoculum of 5.7 x106 spores/ml in International Streptomyces Project 2 (ISP2) medium at neutral pH, incubated at 30°C with horizontal agitation. Phosphorus concentrations revealed that 0.8 g/l of K2HPO4 leaded to optimal growth of the Streptomyces sp. strain MFB28 used. However, bioactive metabolites produced under different phosphorus concentrations were variable according to the bacteria test used. All these results were validated using a statistical test conducted with SPSS version 23.0. | ||
| Keywords | ||
| Streptomyces sp. strain MFB28; Culture condition; Biomass production; Antibacterial activity | ||
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