Biocompatibility and Marginal Adaptability of Bioceramic Putty Material as Root End Filling Material (In Vitro Study) | ||
| Egyptian Dental Journal | ||
| Volume 71, Issue 2 - Serial Number 5, April 2025, Pages 1893-1901 PDF (1.19 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/edj.2025.360205.3383 | ||
| Authors | ||
| Moataz Ahmed Alshafey* 1; Medhat Taha Elfaramawy2; Wael Hamdy El-Shater3 | ||
| 1Endodontic department, Faculty of dentistry, Delta university for science and technology | ||
| 2Endodontic Dep., Faculty of dentistry, Ainshams Univ. | ||
| 3Endodontic Dep, Faculty of dentistry, Ainshams university | ||
| Abstract | ||
| Purpose: This investigation aimed to assess the Biocompatibility and Marginal Adaptability of Bioceramic Putty material as root end filling material compared with Mineral Trioxide Aggregate (MTA). Methodology: For the marginal adaptability test, Thirty-six human extracted single rooted teeth were used. Samples were randomly distributed into 3 groups (n=12) based on the root end filling materials (Cerkamed Bio-MTA, Well-Root Putty, and Gutta percha). The tested materials were mixed and applied in accordance with the manufacturer's instructions. After complete setting, they were immersed into 1% Rhodamine B fluorescent dye for 24h then marginal adaptability was evaluated using confocal laser microscope measuring dye penetration in microns. For biocompatibility test, thirty-six discs with 2 mm thickness and 2 mm diameter were used. The discs were randomly distributed into 3 groups (n=12) consistent with the tested materials. Biocompatibility of the tested samples was measured against hFB (human fibroblast cells) for 24h and 48h using the MTT Cell Viability Assay. Results: For the marginal adaptability test, there were no statistically significant distinctions among Cerkamed Bio-MTA and Well-Root Putty. For biocompatibility test, Well-Root Putty samples showed significant cytotoxicity on both time intervals. Conclusion: Neither Cerkamed Bio-MTA and Well-Root putty showed 100% marginal adaptability but they were close to each other. Cerkamed Bio-MTA and Gutta percha did not affect cell viability whereas; Well-Root Putty showed cytotoxic effect on fibroblastic cells. | ||
| Keywords | ||
| Cerkamed Bio-MTA; Laser confocal Microscope; MTT Assay; Well-Root Putty | ||
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