Biocompatibility and Marginal Adaptability of Bioceramic Putty Material as Root End Filling Material (In Vitro Study)
Alshafey, M., Elfaramawy, M., El-Shater, W. (2025). Biocompatibility and Marginal Adaptability of Bioceramic Putty Material as Root End Filling Material (In Vitro Study). EKB Journal Management System, 71(2), 1893-1901. doi: 10.21608/edj.2025.360205.3383
Moataz Ahmed Alshafey; Medhat Taha Elfaramawy; Wael Hamdy El-Shater. "Biocompatibility and Marginal Adaptability of Bioceramic Putty Material as Root End Filling Material (In Vitro Study)". EKB Journal Management System, 71, 2, 2025, 1893-1901. doi: 10.21608/edj.2025.360205.3383
Alshafey, M., Elfaramawy, M., El-Shater, W. (2025). 'Biocompatibility and Marginal Adaptability of Bioceramic Putty Material as Root End Filling Material (In Vitro Study)', EKB Journal Management System, 71(2), pp. 1893-1901. doi: 10.21608/edj.2025.360205.3383
Alshafey, M., Elfaramawy, M., El-Shater, W. Biocompatibility and Marginal Adaptability of Bioceramic Putty Material as Root End Filling Material (In Vitro Study). EKB Journal Management System, 2025; 71(2): 1893-1901. doi: 10.21608/edj.2025.360205.3383

Biocompatibility and Marginal Adaptability of Bioceramic Putty Material as Root End Filling Material (In Vitro Study)

Egyptian Dental Journal
Volume 71, Issue 2 - Serial Number 5, April 2025, Page 1893-1901  XML PDF (1.19 MB)
Document Type: Original Article
DOI: 10.21608/edj.2025.360205.3383
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Authors
Moataz Ahmed Alshafey email orcid 1; Medhat Taha Elfaramawyorcid 2; Wael Hamdy El-Shaterorcid 3
1Endodontic department, Faculty of dentistry, Delta university for science and technology
2Endodontic Dep., Faculty of dentistry, Ainshams Univ.
3Endodontic Dep, Faculty of dentistry, Ainshams university
Abstract
Purpose: This investigation aimed to assess the Biocompatibility and Marginal Adaptability of Bioceramic Putty material as root end filling material compared with Mineral Trioxide Aggregate (MTA).
Methodology: For the marginal adaptability test, Thirty-six human extracted single rooted teeth were used. Samples were randomly distributed into 3 groups (n=12) based on the root end filling materials (Cerkamed Bio-MTA, Well-Root Putty, and Gutta percha). The tested materials were mixed and applied in accordance with the manufacturer's instructions. After complete setting, they were immersed into 1% Rhodamine B fluorescent dye for 24h then marginal adaptability was evaluated using confocal laser microscope measuring dye penetration in microns. For biocompatibility test, thirty-six discs with 2 mm thickness and 2 mm diameter were used. The discs were randomly distributed into 3 groups (n=12) consistent with the tested materials. Biocompatibility of the tested samples was measured against hFB (human fibroblast cells) for 24h and 48h using the MTT Cell Viability Assay.
Results: For the marginal adaptability test, there were no statistically significant distinctions among Cerkamed Bio-MTA and Well-Root Putty. For biocompatibility test, Well-Root Putty samples showed significant cytotoxicity on both time intervals.
Conclusion: Neither Cerkamed Bio-MTA and Well-Root putty showed 100% marginal adaptability but they were close to each other. Cerkamed Bio-MTA and Gutta percha did not affect cell viability whereas; Well-Root Putty showed cytotoxic effect on fibroblastic cells.
Keywords
Cerkamed Bio-MTA; Laser confocal Microscope; MTT Assay; Well-Root Putty
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