Standardized Melaleuca Leucadendra (L.) L. and Melaleuca Styphelioides Sm. Leaf Extracts Attenuate Obesity in High-Fat-Diet-Fed Rats
Hussein, S., Emam, M., Mansour, D., Saleh, D., Radwan, R., Bakry, S., Hegazi, N. (2025). Standardized Melaleuca Leucadendra (L.) L. and Melaleuca Styphelioides Sm. Leaf Extracts Attenuate Obesity in High-Fat-Diet-Fed Rats. EKB Journal Management System, 68(12), 95-105. doi: 10.21608/ejchem.2025.214538.11265
Sahar A.M. Hussein; Mahmoud Emam; Dina F. Mansour; Dalia O. Saleh; Rasha A. Radwan; Sherien M. Bakry; Nesrine M. Hegazi. "Standardized Melaleuca Leucadendra (L.) L. and Melaleuca Styphelioides Sm. Leaf Extracts Attenuate Obesity in High-Fat-Diet-Fed Rats". EKB Journal Management System, 68, 12, 2025, 95-105. doi: 10.21608/ejchem.2025.214538.11265
Hussein, S., Emam, M., Mansour, D., Saleh, D., Radwan, R., Bakry, S., Hegazi, N. (2025). 'Standardized Melaleuca Leucadendra (L.) L. and Melaleuca Styphelioides Sm. Leaf Extracts Attenuate Obesity in High-Fat-Diet-Fed Rats', EKB Journal Management System, 68(12), pp. 95-105. doi: 10.21608/ejchem.2025.214538.11265
Hussein, S., Emam, M., Mansour, D., Saleh, D., Radwan, R., Bakry, S., Hegazi, N. Standardized Melaleuca Leucadendra (L.) L. and Melaleuca Styphelioides Sm. Leaf Extracts Attenuate Obesity in High-Fat-Diet-Fed Rats. EKB Journal Management System, 2025; 68(12): 95-105. doi: 10.21608/ejchem.2025.214538.11265

Standardized Melaleuca Leucadendra (L.) L. and Melaleuca Styphelioides Sm. Leaf Extracts Attenuate Obesity in High-Fat-Diet-Fed Rats

Egyptian Journal of Chemistry
Article 10, Volume 68, Issue 12, December 2025, Page 95-105  XML PDF (1.61 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2025.214538.11265
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Authors
Sahar A.M. Husseinorcid 1; Mahmoud Emam1; Dina F. Mansourorcid 2; Dalia O. Saleh2; Rasha A. Radwan3; Sherien M. Bakry1; Nesrine M. Hegazi email orcid 1
1Phytochemistry and Plant Systematics Department, Pharmaceutical Industries Institute, National Research Centre, Dokki, Cairo 12622, Egypt.
2Pharmacology Department, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Cairo 12622, Egypt.
3Biochemistry Department, Faculty of Biotechnology, German International University, Regional Ring Rd, East Cairo, New Administrative Capital, Egypt
Abstract
Owing to the increased prevalence of obesity worldwide, and the well-known side effects of the commercially available drugs, there is a pressing need for re-exploring our natural resources for safer alternatives. Aim of the study: The crude extracts of the leaves of two common Egyptian Melaleuca species (Melaleuca leucadendra, ML and Melaleuca styphelioides, MS) were tested for their in vitro α-amylase inhibition and in vivo anti-obesity potential in a high fat-fed (HFD) rat model. Materials and methods: For the standardization of both extracts, qualitative and quantitative metabolite fingerprinting was performed via high-performance liquid chromatography with diode array detection (HPLC/ DAD) and NMR-based metabolomics. Results: Both extracts were characterized by the presence of phenolic acids with gallic and ellagic acids as the major ones. Besides, some flavonoids were detected such as rutin, naringenin, daidzein, quercetin, apigenin, and kaempferol. The extracts showed 85 %± 3.5 and 83 % ± 3.6 reductions in the α-amylase activity at 600µg/ml with an IC50 347 ± 4.6 and 365 ± 3.8µg/ml, for ML and MS respectively. Both extracts displayed evident anti-obesity potential by attenuating HFD-induced weight change, lipid profile, and adipose tissue markers of obese rats. Conclusion: Melaleuca extracts are potential candidates for attenuating obesity by reducing the gain in body weight and modulating the lipid profile and adipose tissue markers, in a dose-dependent approach.
Keywords
Adipose tissue markers; HPLC/DAD; lipid profile; Melaleuca; obesity
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