EFFECTS OF VACUOLAR TRANSPORTER CHAPERONE 1 DISRUPTION ON PHOSPHORUS STRESS RESPONSE NETWORK IN CHLAMYDOMONAS REINHARDTII | ||||
| Journal of Biological Chemistry and Environmental Sciences | ||||
| Volume 14, Issue 1, March 2019, Page 89-109 PDF (899.04 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bces.2025.425037 | ||||
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| Abstract | ||||
| Phosphorus (P) stress response network and transport has been studied in microalgae. However, less is known about the interactions between polyphosphate (polyP) biosynthesis and P stress response network. Also, knowledge about polyP metabolism in microalgae remains limited. In this study, the molecular mechanisms of polyP biosynthesis and its link with P stress response regulation and phosphate (Pi) transport were investigated in Chlamydomonas reinhardtii using a mutant defective in the vacuolar transporter chaperone 1 gene (VTC1). The presence of polyP was abundant in the C. reinhardtii VTC1 rescue strains when Pi was copious, whereas polyP was degraded when P was depleted in the medium. Also, deletion of VTC1 severely repressed the expression (20- fold compared to the expression in VTC1 rescue) of Phosphorus Stress Regulator 1 (PSR1) as well as Phosphate Transporter Type B2 (PTB2) and PTB4, suggesting that defects of VTC1 may perturb the P stress response through repression of PSR1 expression. Moreover, differentiated expressions of type A (acidic phosphate transporters) and type B (alkaline phosphate transporters) are observed in response to P deprivation. These EFFECTS OF VACUOLAR TRANSPORTER CHAPERONE 1 DISRUPTION90 data suggest that PTBs in C. reinhardtii, grown in neutral to slight basic medium pH conditions, may be the primary Pi transporters under P deprivation and they are controlled by PSR1 which is may be regulated by polyP molecules. Thus, manipulation of polyP biosynthesis under P stress condition may be a promising strategy for agriculture in alkaline soil to face and tolerate P deprivation stress. | ||||
| Keywords | ||||
| Key words: Chlamydomonas reinhardtii; vacuolar transporter chaperone 1; polyphosphate; phosphorus stress response regulators; phosphate transporters; alkaline soils | ||||
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