EFFECTS OF VACUOLAR TRANSPORTER CHAPERONE 1 DISRUPTION ON PRODUCTION OF TRIACYLGLYCEROLS QUALITY AND QUANTITY IN CHLAMYDOMONAS REINHARDTII UNDER PHOSPHORUS DEPRIVATION | ||
| Journal of Biological Chemistry and Environmental Sciences | ||
| Volume 14, Issue 1, March 2019, Pages 111-133 PDF (854.54 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/bces.2019.425038 | ||
| Abstract | ||
| Microalgal lipid biosynthesis has been extensively studied under nitrogen depleted conditions where triacylglycerols (TAGs) rapidly accumulate while cell growth is arrested. However, less is known about TAG production under phosphorus depleted conditions. Also, available knowledge about polyphosphate metabolism in microalgae remains limited. In this study, the molecular mechanisms of polyphosphate biosynthesis and its link with the lipid biosynthesis pathway are investigated in Chlamydomonas reinhardtii using a mutant defective in the vacuolar transporter chaperone 1 gene (VTC1). The presence of polyphosphate was abundant in the C. reinhardtii VTC1 rescue strain when phosphorus was copious, whereas polyphosphate was degraded when phosphorus was depleted in the medium. In addition, deletion of the VTC1 gene enhanced TAGs production under phosphorus depleted conditions, resulting in a 9.6-fold increase in TAGs concentration at day 21. A defect in the VTC1 gene also affected the fatty acid composition of EFFECTS OF VACUOLAR TRANSPORTER CHAPERONE 1 DISRUPTION112 TAGs, promoting accumulation of mono- and poly-unsaturated fatty acids at the expense of saturated fatty acids. These data suggest a negative relationship between polyphosphate biosynthesis and TAG biosynthesis in microalgae, and provide a new strategy to improve the quality and yield of algal biofuels through manipulation of polyphosphate biosynthesis. | ||
| Keywords | ||
| Key words: Chlamydomonas reinhardtii; vacuolar transporter chaperone 1; phosphorus deprivation; polyphosphate; triacylglycerols | ||
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