STUDIES ON PROPAGATION OF ACIDANTHERA MURIELAE PLANTS BY USING TISSUE CULTURE TECHNIQUE | ||||
| Journal of Biological Chemistry and Environmental Sciences | ||||
| Volume 14, Issue 1, March 2019, Page 135-150 PDF (458.38 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bces.2019.425045 | ||||
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| Abstract | ||||
| This study was intended to find out a well-defined commercial production protocol for in vitro propagation of Acidanthera murielae. In this respect, buds from Acidanthera corms were effectively surface sterilized using sodium hypochlorite solution at 2% for 20 min. MS medium supplemented with 2 mg/l IBA (Indole Butyric Acid) was the best medium for shoot length and number of leaves promotion during the establishment stage. For further multiplication, MS medium supplemented with 1 mg/l BA and 2 mg/l Kin formed the highest number of shoots. For in vitro rooting, MS medium at half strength supplemented with 2.0 mg/l IBA was suitable for root formation. The plantlets were transferred to a mixture of peatmoss:sand at a ratio of 1:1 with great success for ex vitro acclimatization inside the greenhouse conditions. | ||||
| Keywords | ||||
| Key words: Acidanthera; Cormel; In vitro; Micropropagation; Peacock Orchid; Shoot tips; Tissue culture | ||||
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