BIOCHEMICAL STUDIES ON ANTIBIOTIC RESISTANT AND VIRULENCE GENES OF PSEUDOMONAS AERUGINOSA, ISOLATED FROM MASTITIC COWS' MILK AND ITS ALTERATION ON BLOOD BIOCHEMICAL PARAMETERS | ||||
| Journal of Biological Chemistry and Environmental Sciences | ||||
| Volume 14, Issue 1, March 2019, Page 175-195 PDF (581.64 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bces.2019.425057 | ||||
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| Abstract | ||||
| The emerged antibiotic resistant bacteria issue is considered as an important problem that threats human's life including; health and agriculture affairs. Pseudomonas aeruginosa is one of the bacteria that affecting milk production and responsible for mastitis in cattle. Out of 50 mastitic milk samples, only 6 samples were found to be positive for Pseudomonas aeroginousa with an incidence of 12%. The PCR detection of Pseudiomonas aeroginosa antibiotic resistance gene blaVIM and the virulence gene toxA, indicated that only 4 isolates showed to be positive for blaVIM gene. While, all the 6 positive milk Pseudomonas aeroginousa isolates showed to be positive for toxA gene. The antibiotic sensitivity results showed that the isolates were resistant to Tetracycline, Piperacillin and Oxacillin and Piperacillin. However, these isolates were sensitive to Amikacin, Ceftazidime Cefoperazone and Impenem. Also, the infected animal serum biochemical parameters when compared to the healthy uninfected animals (control group), showed an elevation in the values of total protein, globulin, triglyceride, alanine aminotransferase, aspartate aminotransferase, uric acid and creatinine. While it showed decline in the values of albumin, glucose, cholesterol, low density lipoprotein, and high density lipoprotein. | ||||
| Keywords | ||||
| Key words: Antibiotic resistance genes; Blood parameters; PCR; Pseudomonas aeruginosa; Mastitic cows and Virulence genes | ||||
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