IMMUNOHISTOCHEMICAL STUDY ON SCHISTOSOMA MANSONI INDUCED LIVER FIBROSIS IN EXPERIMENTALLY INFECTED MICE

ELFARAMAWY, MOHAMMED S.; ELBEHERY, AHMED A. E.; AHMAD, HAITHAM KH.; MAZEN, MOAMEN A. I.; AMIN, MOHAMED A.

doi:10.21608/jesp.2025.425077

	IMMUNOHISTOCHEMICAL STUDY ON SCHISTOSOMA MANSONI INDUCED LIVER FIBROSIS IN EXPERIMENTALLY INFECTED MICE
Journal of the Egyptian Society of Parasitology
Article 16, Volume 55, Issue 1, April 2025, Page 129-136 PDF (778.22 K)
Document Type: Original Article
DOI: 10.21608/jesp.2025.425077
View on SCiNiTO
Authors
MOHAMMED S. ELFARAMAWY¹; AHMED A. E. ELBEHERY¹; HAITHAM KH. AHMAD²; MOAMEN A. I. MAZEN²; MOHAMED A. AMIN³
¹Departments of Parasitology, Faculty of Medicine Al-Azhar University, Cairo, Egypt
²Departments of Parasitology, Faculty of Medicine Al-Azhar University, Assuit, Egypt
³Department of Microbiology and Immunology
Abstract
In chronic schistosomiasis, eggs trapped in the liver triggered granulomatous immuno pathological lesions which leading to severe liver fibrosis. The study checked the impact of IL 17A on serum IL-13 level, TNF-α and TGF-1β protein expressions on S. mansoni induced liver fibrosis in experimentally infected mice. Forty four male C57BL/6 pathogen-free mice obtained, divided into 4 groups: GI negative control, GII positive control infected with 30±5 cercariae, GIII infected/anti-IL-17 mAb was given, GIV isotype-matched IgG2a mAb was given as a chal lenge, were sacrificed at 6, &10 weeks postinfection, then underwent liver enzymes and cyto kines assessment, histopathological and immunohistochemical studies. The results showed that the profibrogenic activity of IL 17 was blocked by anti/IL-17 monoclonal antibodies with signif icant decrease in IL 13, liver enzymes, pro-inflammatory cytokines and granuloma size. Also, reduced TGF- β1 expression, and increase TNF-α expression levels in livers of S. mansoni infected mice in the later stages, inducing its anti-fibrotic effects. So may be targeted in Schisto soma egg induced liver fibrosis as potential immunotherapeutic agent.
Keywords
Schistosoma mansoni; liver fibrosis; IL-17; cytokines; and immunohistochemistry


Statistics Article View: 96 PDF Download: 43