Potential Therapeutic Impact of Platelet Rich Plasma (PRP) Versus Exosomes on Testicular Ischemia Reperfusion Injury in Adult Albino Rats: A Histological Study | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 30 April 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2025.369832.2238 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Zeinab Hegab1; Sahar Gamal Abo Elfadl2; Mai Mostafa Hamed Shaker   1; Amal Ahmed Farag 3
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| 1Histology Department, Kasr Alainy Faculty of Medicine Cairo University | ||||
| 2Histology Department, Faculty of Medicine, Cairo University and Misr University for Science and Technology | ||||
| 3Department of histology, kasr el ainy medical school, Cairo University | ||||
| Abstract | ||||
| Background: The current work targeted to assess and compare the protective impact of platelet-rich plasma (PRP) versus mesenchymal stem cell-derived exosomes (EXO) on ischemia reperfusion (I/R) injury of the testis in an experimental representative of adult albino rat using biochemical, histological and morphometric studies. Materials & Methods: The animals (n=48) were classified into: Donor Group, (N=4) for PRP preparation; GI (Sham-operated control group, N=14); GII Torsion-Detorsion group (TD group N=4) subjected to bilateral testicular torsion followed by detorsion and sacrificed after 2hours; GIII (Spontaneous recovery group, N=8) with no further intervention following TD injury; Group IV (PRP-injected group, N=8) and Group V (EXO-injected group, N=10). 2 rats of GV were sacrificed after 48 hrs to confirm homing of EXOs. Half the rats of GIII, GIV & GV were surrendered 1 week following TD, other half of rats 4 weeks following TD with their control subgroups. Results: Increased serum follicular-stimulating hormone (FSH), malondialdehyde (MDA) and Caspase-3, decreased Testosterone and reduced-glutathione (GSH) were recorded in GII versus GI. FSH increased in GIII versus GI then decreased in GIV and GV. Testosterone and GSH decreased in GIII, GIV and GV and increased in GIV and GV versus GIII. Oxidative stress markers (MDA and Caspase-3) increased in GIII, GIV and GV versus GI, but dropped in GIV and GV versus GIII. Morphological inflammatory changes were detected in GII, that progressed in GIII and improved in GIV and GV. Degenerative changes as reduced thickness of spermatogenic epithelium, basement membrane impairment and apoptosis were noticed in GIII then regressed in GIV and GV. Conclusion: I/R-induced inflammatory morphological changes progressed to degenerative structural and functional alterations if left with no effective intervention. MSCs-derived EXO proved an ameliorating effect more pronounced than that of PRP in short duration. While PRP proved a remarkable ameliorating effect after longer periods. | ||||
| Keywords | ||||
| IR; PRP; EXOs; MDA; PCNA | ||||
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