Study of Cyclooxygenase-2 (A-1195G) and Matrix Metalloproteinase-2 (C-1360T) Genes Single Nucleotide Polymorphisms in Association with Colorectal Carcinoma | ||||
| Ain Shams Medical Journal | ||||
| Volume 76, Issue 1, March 2025, Page 95-108 PDF (538.04 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/asmj.2025.309745.1293 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ghada Maher Thabet  1; Samar Saad2; Mohamed Salama Mohamed3; Nahla Aly Fawzy2; Nevine Ezzeldin ElAbd2
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| 1Department of Clinical and Chemical Pathology Faculty of medicine-Cairo university | ||||
| 2Clinical and Chemical Pathology Department, Faculty of Medicine-Cairo University, Cairo, Egypt | ||||
| 3Surgical Oncology Department, National Cancer Institute, Cairo University, Cairo, Egypt | ||||
| Abstract | ||||
| Background: Colorectal cancer (CRC) is the third most common type of cancer and the second leading cause of cancer-related death in the world, with around 900,000 deaths per year. COX-2 expression is associated with MMP-2 expression in numerous malignancies, suggesting that MMPs are linked to COX-2-mediated carcinogenesis. The growth of tumors, specifically colorectal cancer (CRC), has been linked to various single nucleotide variants (SNV) in the MMP2 promoter region, which is involved in the process of oncogenesis. The aim of this study: Was to evaluate the risk of COX-2 (A/G transition at -1195) and MMP-2 (C/T transition at -1306) single nucleotide variants, with respect to CRC susceptibility in Egyptian patients and whether these genetic variants are associated with the clinical and laboratory features of the disease. This study was carried out on 200 subjects (100 confirmed colorectal carcinoma patients and 100 healthy subjects served as a control group), both groups were age and sex matched. CRC patients and control subjects were subjected to determination of Matrix metalloproteinase-2 (rs243865) and cyclooxygenase-2 (rs68465) single gene variants using TaqMan Real-Time PCR. Results: Of the present study revealed significant association between the presence of risky allele (T) in CT genotype of MMP-2 with both lymph node metastasis (p=0.008) and invasive stage of CRC (P=0.018). Also, there was a significant association between the presence of risky allele (G) in AG and GG genotypes in COX-2 and site of CRC in colon (P=0.004). Conclusions: MMP-2 (rs243865) variant increase the risk of invasion of colorectal cancer. | ||||
| Keywords | ||||
| Cyclooxygenase-2; colorectal carcinoma; matrix metalloproteinase-2; variant | ||||
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