Surveillance and Molecular Fingerprinting of Multidrug-Resistant Pseudomonas aeruginosa Strains from Clinically Diseased Companion Animals and Mastitic Cows | ||||
| Egyptian Journal of Veterinary Sciences | ||||
| Articles in Press, Corrected Proof, Available Online from 07 May 2025 PDF (898.39 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejvs.2025.367731.2698 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Amal Hassanien  1; Eman I, Shafeek2; Mahmoud Hashad 3
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| 1microbiology department faculty of vetriniry medicine cairo university Giza Egypt | ||||
| 2Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt | ||||
| 3Professor of Microbiology, Faculty of Veterinary Medicine, Cairo University | ||||
| Abstract | ||||
| Pseudomonas aeruginosa is a public health threat that affects various species, causing various clinical infections. This study investigated the occurrence and diversity of MDR P. aeruginosa in different samples. One hundred samples were collected from specimens of different clinically ill animal species. Bacteriological investigation resulted in the recovery of 64 P. aeruginosa isolates (64%) identified phenotypically and genotypically. The antimicrobial susceptibility test indicated 100% susceptibility for ciprofloxacin and levofloxacin. The susceptibility to moxifloxacin, clarithromycin, tetracycline, ceftazidime, ceftriaxone, and cefotaxime were conferred by 93.75%, 40.62%, 21.87%, 15.62%, 12.5% and 3.12% of the isolates, respectively. It is not worth mentioning that all isolates were resistant to ampicillin, sulbactam/ampicillin, erythromycin, nitrofurantoin, and meropenem, which belong to four different antimicrobial groups, denoting their being multidrug-resistant isolates. BamH1 restriction digestion of 16S rRNA PCR product (1500 bp) revealed two uniform segments (650 and 850 bp) in all isolates. Photogenically, Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR) was applied on 20 isolates, and they were categorized into three main clusters with 50% similarity. In conclusion, P. aeruginosa is a diverse microorganism with great MDR patterns, which is considered a danger bell for public health. | ||||
| Keywords | ||||
| Pseudomonas aeruginosa; PCR; BamH1; ERIC-PCR; MDR | ||||
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