GENETIC IDENTIFICATION OF SOME EGYPTIAN VICIA FABA L. CULTIVARS AND THEIR RELATION TO OROBANCHE CRENATA L. TOLERANCE | ||||
| Journal of Biological Chemistry and Environmental Sciences | ||||
| Volume 3, Issue 1, March 2008, Page 123-140 PDF (807.23 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bces.2008.426656 | ||||
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| Abstract | ||||
| Ten Egyptian faba bean cultivars were assessed for Orobanche tolerance in a field trial during the winter season 2002-2003. Two traits related to Orobanche infection were estimated i.e., number of spikes/plant and weight of Orobanche stems/plant. Statistical analysis revealed differences among the studied cultivars in relation to Orobanche tolerance/susceptibility. The results confirmed that Giza 843 and Misr1 were the most tolerant cultivars, while Giza 643, Giza716 and Giza717 were the most susceptible ones. SDS-PAGE and RAPD analysis were used to study the genetic relationships among the ten faba bean cultivars and to detect some molecular genetic markers for Orobanche tolerance /susceptibility. Both SDS- PAGE and RAPD analysis revealed distinctive bands that successfully determined tolerant from susceptible bean cultivars. Data analyses for both SDS-PAGE and RAPD-PCR were used to construct a dendrogram showing the genetic relationships among the faba bean cultivars and fitted well with their pedigrees. | ||||
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