EFFECT OF G-CSF AS A NOVEL MODULATOR ON ACUTE BLOOD GLUCOSE FLUCTUATION INDUCED ENDOTHELIAL INJURY IN RAT AORTA: HISTOLOGICAL, IMMUNOHISTOCHEMICAL AND BIOCHEMICAL STUDY | ||||
| Egyptian Journal of Histology | ||||
| Articles in Press, Accepted Manuscript, Available Online from 10 May 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2025.357183.2208 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Safaa Said Hassan  1; Amal Taha Abou-Elghait2; Ragaa H. Salama3; Esam Omar Kamel4; Abdalla Shehatah Sayed4
| ||||
| 1Histology Department, Faculty of Medicine, Assiut University, Assiut, Egypt Histology Department, Merit University, New Suhag City, Suhag, Egypt | ||||
| 2Histology Department, Faculty of Medicine, Assiut University, Assiut, Egypt b Histology Department, Sphinx University, New Assiut City, Assiut, Egypt | ||||
| 3Medical Biochemistry Department, Faculty of Medicine, Assiut University, Assiut, Egypt | ||||
| 4d Histology Department, Faculty of Medicine, Al-Azhar University, Assiut, Egypt | ||||
| Abstract | ||||
| Background/aims. Blood glucose continuously fluctuates in healthy individuals. This study compared the risk of acute glucose fluctuation and constant hyperglycemia in the aorta of non-diabetic animal models. The possible therapeutic effect of granulocyte-colony stimulating factor (G-CSF) was investigated. Methods. Thirty male Wistar rats were divided into (Gr-1) control, (Gr-2) constant hyperglycemia (Gr-2), (Gr-3) acute glucose fluctuating induced by injection of 1 ml 50% glucose solution intraperitoneal every 4 hours for 4 days, (Gr-4) Recovery group: like (Gr-3) but did not receive anything for 7 days then sacrificed and (Gr-5) treated group. After induction of glucose fluctuation, rats were injected with 200 microgram/kg/day of diluted G-CSF (Neupogen®) subcutaneously for 5 days. Blood levels of glucose, Insulin and C-peptide were measured. The aorta was examined histologically by (Hematoxylin & Eosin, Masson and Orcein), immunohistochemically by Caspase-3 and VEGF markers and by electron microscope. Results: Glucose returned to normal in Gr-4 and Gr-5. Insulin levels decreased in Gr-2, Gr-3, Gr-4 and Gr-5. C-peptide decreased in Gr-2 and Gr-3. Gr-4 and Gr-5 showed no significant decrease in C-peptide. Gr-3 showed endothelium damage, destructed mitochondria, an increase in collagen and disrupted elastic lamellae. Gr-2 showed less damage than Gr3. Gr-4 showed limited structural improvement. Gr-5 showed normal cell proliferation .Gr-3 showed an increase in the amount of collagen deposition while Gr-4 and Gr-5 showed significant decrease in collagen. The elastic lamellae in Gr-2 and Gr-3 were disrupted and irregular, while in Gr-4 and Gr-5 were regularly organized . Caspase-3 expression was weak in the endothelium of Gr-2 and Gr-3 and strong in smooth muscles while Gr-4 and Gr-5 showed decreased expression. VEGF expression was weak in Gr-1, strong in Gr-3, strong in endothelium weak in smooth muscle in Gr-2, and weak in Gr-4 and Gr-5. Conclusion: AGF causes more aortic damage. G-CSF ameliorate these changes. | ||||
| Keywords | ||||
| Endothelium; AGF; CHG; G-CSF | ||||
|
Statistics Article View: 86 |
||||
