Using Local Estrogen with Implication of Modified Guillotine Technique to Improve Regenerative Process of pPartially Resected Liver in Rabbits | ||||
| Journal of Applied Veterinary Sciences | ||||
| Article 3, Volume 10, Issue 3, July 2025, Page 15-23 PDF (693.9 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/javs.2025.370220.1564 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Al Kattan L. M.   ; Salah Aldeen M. S.; Martena H.A.
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| Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq | ||||
| Abstract | ||||
| This research planned to determine the effect of local estrogen on the regeneration of partially hepatectomized rabbits. Twelve male rabbits were included in the present study, hereby allocated randomly into two equal groups. In the control group, partial hepatectomy was performed with the modified guillotine technique. In the treatment group, the steps are similar to the control (included partial hepatectomy) with the application of estrogen (0.2 mg). Macroscopic, laparoscopic, histopathological and immunohistochemical changes were recorded at 1, 2, and 4 weeks. Clinically, all rabbits survived a long period of experiment. Grossly, in the second group, 7 days post-surgery, there were no signs of inflammation and no intraoperative fluid. The results of laparoscopy 30 days in the control group indicated a change in liver color with severe adhesion between the liver and viscera; in the treatment group, the results indicated the presence of mild adhesion between the liver and viscera and normal liver structure and color. Histopathological results at 7 days in the control group showed cell swelling, congestion, and inflammatory cells, whereas the results at 30 days exhibited mild congestion of sinusoid, binucleate hepatocyte and fibrosis. In the treatment group, the results at 7 days indicated the presence of normal hepatocytes, fibrosis, and mild congestion in the sinusoid. At 30 days, results indicated the presence of normal hepatocytes, sinusoid and central veins. Immunoreactivity of tumor necrosis factor alpha (TNF-α) in the control group at 14 days indicated high expression, indicated as brown granules in hepatocytes, whereas in the treatment group at 14 days the expression was very mild as brown granules in hepatocytes. Based on laparoscopic, histopathological and immunohistochemistry changes, the application of estrogen on the hepatectomized site is beneficial and feasible to improve the regenerative process of the liver in rabbits. | ||||
| Keywords | ||||
| Estrogen; Modified guillotine; Partial hepatectomy; Rabbit; TNF-α | ||||
| References | ||||
|
ABED, F.M, RAHAWI, A.M, AL-SABAAWY, H.B., DARK, M. J., and ABDULJAWAAD, A.N., 2023. Pathological and Immunohistochemical Findings of Prostate Glands from Clinically Normal Dogs. American J of Anim. and Vet. Scie.18(4), 317–326. https://doi.org/10.3844/AJAVSP.2023.317.326.
ALBADRANY, M.S. 2009.Laparoscopic ovariectomy in rabbits. Iraqi J of Vet. Sci. 23(2) (51-55) https://doi.org /10.33899/ijvs.2009.5733
ALHAMDANY, M.S., and ALKATTAN, L.M., 2019. Laparoscopic and hand-assisted cystorrhaphy strengthened with omental pedicle in dogs: Radiographic and ultrasonographic. Iraqi J Vet Sci. 33(2):347-352. https://doi.org/ 10.33899/ijvs.2019.163026.
ALHEANI, W. A., and AL-KENNANY, E., 2013. Partial Hepatectomy Achievement by New Device in Rabbits Model. AL-Qadisiya J. of Vet.Med.Sci. 12 (1):6-11. https://doi.org/ 10.13140/RG.2.2.27147.52008
AL-KADHIMY, B. J., and GHANI, B.A., 2015. In vivo histological evaluation of the effect of the topical application of estrogen hormone on wounds healing in ovariectomized rabbit. J Bagh Coll Dent. 27(1):100-4. jbcd.uobaghdad.edu.iq/index.php/jbcd/article/view/642
ALKATTAN, L.M., ALHASAN, H.M., and ALBADRANY, M.S., 2014. Laparoscopic nephrectomy in Iraqi cat. Iraqi J Vet Sci. 28(1);17-20. https://doi.org/ 10.33899/ijvs.2014.89466.
ATIYAH, A.G., and ALKATTAN, L.M., 2024. Impact of Fabricated Coral Shell Hydroxyapatite Powder and Autologous Plasma Rich- fibrin in Remodeling of the Mandibular Bone Critical Size Defect in Dogs: Histopathological and Immunohistochemical Study. Journal of Applied Veterinary Sciences, 9 (2): 111-119. https://dx.doi.org/10.21608/javs.2024.266431.1312
ASHCROFT, G.S., JEONG, M.J., ASHWORTH, J.J., HARDMAN, M., JIN, W., MOUTSOPOULOS, N., WILD, T., MCCARTNEY-FRANCIS, N., SIM, D., MCGRADY, G., SONG, X.Y., and WAHL, S.M., 2012.Tumor necrosis factor-alpha (TNF-α) is a therapeutic target for impaired cutaneous wound healing. Wound Repair Regen. 20(1):38-49. https://doi.org/10.1111/j.1524-475X.2011.00748. x.
ASHCROFT, G.S., MILLS, S.J., LEI, K., GIBBONS, L., JEONG, M.J., TANIGUCHI, M., BUROW, M., HORAN, M. A., WAHL, S.M., and NAKAYAMA, T., 2003. Estrogen modulates cutaneous wound healing by downregulating macrophage migration inhibitory factor. J Clin Invest. 111(9):1309-18. https://doi.org/ 10.1172/JCI16288.
ASHCROFT, G.S., GREENWELL-WILD, T., HORAN, M A., WAHL S.M., and FERGUSON M., 1999. Topical estrogen accelerates cutaneous wound healing in aged humans associated with an altered inflammatory response. Am J Pathol.;155(4):1137-46. https://doi.org/10.1016/S0002-9440(10)65217-0.10.
BATMUNKH, B., CHOIJOOKHUU, N., SRISOWANNA, N., BYAMBATSOGT, U., SYNN, O.P., NOOR, A.M., YAMAGUCHI, Y., and HISHIKAWA, Y., 2017. Estrogen Accelerates Cell Proliferation through Estrogen Receptor α during Rat Liver Regeneration after Partial Hepatectomy. Acta Histochem. Cytochem., 50(1):39-48. https://doi.org/ 10.1267/ahc.17003.
BHAVSAR, B., JANSSON-KNODELL, C. L., GILMORE, A.C., and CRABB, D.W., 2021.The role of nutrition in alcohol-associated liver disease. Review article: Aliment. Pharmacol. Ther. 53: 1268–1276. https://doi.org/10.1111/apt.16380.
BÜRGISSER, G.M, EVROVA, O., HEUBERGER, D.M, RIEBER, J., GIOVANOLI, P., CALCAGNI, M., and BUSCHMANN, J., 2021. Delineation of the healthy rabbit liver by immunohistochemistry. A technical note Acta Histochemica. 151795. https://doi.org/10.1016/j.acthis.2021.151795.
DRUDE, N., PAWLOWSKY, K., TANAKA, H., and FUKUSHIMA, K., 2016. ¨gel BK2 and Tolba R H Severity assessment in rabbits after partial hepatectomy: Part I Laboratory Animals. 50(6) 468–475 https://doi.org/10.1177/0023677216677949
EAGON, P. K, PORTER, L. E, FRANCAVILLA, A., DILEO, A., and VAN THIEL, D.H., 1985. Estrogen and androgen receptors in liver: their role in liver disease and regeneration. Seminars in Liver Disease,5(1), 59–69. https://doi.org/10.1055/S-2008-1041758.
IDRISS, H.T., and NAISMITH, J.H., 2000. TNF alpha and the TNF receptor superfamily: structure-function relationship(s). Microsc Res Tech.1;50(3):184-95. https://doi.org/10.1002/1097-0029(20000801).
JANG, D.I., LEE, A.H., SHIN, H.Y., SONG, H.R., PARK, J.H, KANG, T.B., LEE, S.R., and YANG, S.H., 2021.The role of Tumor Necrosis Factor Alpha (TNF-α) in Autoimmune Disease and Current TNF-α Inhibitors in Therapeutics. Int J Mol Sci. 8;22(5):2719. https://doi.org/10.3390/ijms22052719.
JEONG, H., LEE, C.H., LEE, M. J., and JUNG, Y., 2023. Therapeutic strategies to improve liver regeneration after hepatectomy. Experimental Bio.and Medi. 248: 1313–1318. https://doi.org/10.1177/1535370223119119.
KHAN, D., and ANSAR, A.S., 2016. The immune system is a natural target for estrogen action: Opposing effects of estrogen in two prototypical autoimmune diseases. Front. Immunol. 6, 635. https://doi.org/10.3389/fimmu.2015.00635
KILIMCI, F.S. 2020. Rabbit Liver Lobes: An Anatomical Study of Experimental Surgical ApproachesErciyes. Üniv Vet Fak Derg. 17(2): 103-108. https://doi.org/10.32707/ercivet.760679.
LI, Y.C, CHAO, A., YANG, L. Y., HUANG, H.Y., HUANG, Y.T., KUO, H.H., and WANG, C.J., 2018. Electrothermal bipolar vessel sealing device (LigaSure) versus conventional diathermy in laparoscopic myomectomy: A propensity-matched analysis. Plos One. 13: e193611.
LUNA, L.G. 1968. Manual of histologic staining methods of the armed forces institute of pathology. 3rd ed. USA: McGraw-Hill; 18-20 p.
MUKAI, K., NAKAJIMA, Y., ASANO, K., and NAKATANI, T., 2019.Topical estrogen application to wounds promotes delayed cutaneous wound healing in 80-week-old female mice. PLoS ONE. 14(11): e0225880 https://doi.org//10.1371/journal. pone.0225880
NEGASEE, K.A. 2021. Hepatic Diseases in Canine and Feline: A Review. Vet. Med. Open J.6(1), 22–31. https://doi.org/10.17140/VMOJ-6-155.
OLTHOF, P.B, HEGER, M., VAN LIENDEN, K.P, DE BRUIN, K., BENNINK, R.J., and VAN GULIK, T.M., 2017. Comparable liver function and volume increase after portal vein embolization in rabbits and humans. Surgery. 161 (3):65. https://doi.org/ 10.1016/j.surg.2016.08.039
PÁRAMO, M., GARCÍA-BARQUIN, P., SANTA MARÍA, E., MADRID, J. M., CABALLEROS, M., BENITO, A., SANGRO, B., INARRAIRAE, G.U.I.M., and BILBAO, J.I., 2017.Evaluation of the rabbit liver by direct portography and contrast-enhanced computed tomography: anatomical variations of the portal system and hepatic volume quantification. Eur Ra diol Exp. 1(1):1- 7. 8-65. https://doi.org/ 10.1186/s41747-017-0011-8
RAJABI, M.A., and RAJABI, F., 2007.The effect of estrogen on wound healing in rats. Pakstan. J Med Sci. 23(3): 349-35. https://www.researchgate.net/publication/275653944_
TAO, Z.Z., CHU, Z.Q., JIANG, J.B., and JI, Q., 2021.Study of R2 value to evaluate regulation of liver regeneration by hepatic warm ischemia-reperfusion injury after partial hepatectomy in rabbits. Zhonghua Gan Zang Bing Za Zhi ., 29(6):539-544. https://doi.org/ 10.3760/cma.j.cn501113-20191214-00461.
TREFTS, E., GANNON, M., and WASSERMAN, D.H., 2017.The liver. R1147-r1151 Curr. Biol. 27 (21), 1147. https://Doi.org/10.1016/j.cub.2017.09.019.
TÜZÜN, S., ÇAKIR, M., SAVAŞ, O.A., 2015.Tatar C. Hepatocellular carcinoma-liver resections. Haseki Tıp Bülteni.53(1):1-9. https://doi.org/10.4274/HASEKI.1999.
VACHLIOTIS, I. D., and POLYZOS, S.A., 2023.The Role of Tumor Necrosis Factor-Alpha in the Pathogenesis and Treatment of Nonalcoholic Fatty Liver Disease". Current Obesity Reports.12 (3): 191–206. https://doi.org/10.1007/s13679-023-00519-y.
VOBODA, R.M., DEL, ROSSO, J. Q., ZEICHNER, J. A., and DRAELOS, Z.D., 2018. Revisiting the Beneficial Effects of Estrogen on the Skin: A Comprehensive Review of the Literature and a Look to the Future. SKIN.The J of Cutan. Med. 2, 5. https://doi.org/10.25251/2.3.4.
WU, Y., LI, N., SHU, X., LI, W., ZHANG, X., LÜ, D., and LONG, M., 2023. Biomechanics in liver regeneration after partial hepatectomy. Front. Bioeng. Biotechnol. 11:1165651. https://doi.org/10.3389/fbioe.2023.1165651
YOSHIDA, H., TANIAI, N., YOSHIOKA, M., HIRAKATA, A., KAWANO, Y., SHIMIZU, T., UEDA, J., TAKATA, H., NAKAMURA, Y., and MAMADA, Y., 2019.Current status of laparoscopic hepatectomy. J Nippon Med Sch.; 86:201-206. https://doi.org/ 10.1272/jnms.JNMS.2019_86-411
YOVCHEVA, K.S., DIMITROV R., and YOVCHEV, D., 2012. Macromorphological features of the liver in domestic rabbit (ORYCTOLAGUS CUNICULUS). Traka J of science.10(2). https://api.semanticscholar.org/CorpusID:28616149.
ZEDAN, I. A., ALKATTAN, L.M., and AL-MAHMOOD, S.S., 2025. Immunological and histopathological changes after topical application of aloe Vera leaf extract during ventral hernioplasty in rams. Assiut Vet. Med. J. 71 (184):376-3858. https://doi.org//10.21608/avmj.2025.308871.1331
ZHANG, H., WANG, J., CAO, Y., ZHANG, Z., SHEN, J., TONG, J., and WANG, H., 2020. Laparoscopic left hemi hepatectomy in small dogs: an easy and effective new technique. Acta Vet. Brno.89: 367–373 https://doi.org//10.2754/avb202089040367
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