Frequency of methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin resistance (VRSA) among S. aureus isolated from different clinical specimens in Thi-Qar Province | ||
| Microbes and Infectious Diseases | ||
| Articles in Press, Accepted Manuscript, Available Online from 12 May 2025 | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mid.2025.371565.2657 | ||
| Authors | ||
| Saif Sadoon Hussein* ; Saad Abdul Azeez Atiayh | ||
| Department of Microbiology, College of Medicine, University of Thi-Qar, Thi-Qar, 64001, Iraq | ||
| Abstract | ||
| Background Staphylococcus aureus are Gram-positive, catalase-positive cocci from the Staphylococcaceae family. These facultative anaerobes often form clustersand lacking motility and spore formation. S. aureus is resistant to extreme circumstances like high salt concentration and osmotic stress. This study aimed to identify the frequency of methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S aureus (VRSA) among the S. aureus isolates obtained from different clinical sources. Methods: From September 2023 to January 2024, this research was carried out at AL-Hussein Teaching Hospital in the Iraqi province of Thi-Qar. A total of 523 clinical specimens were obtained from diverse sources, including (Nasal, Urine, Wound, Burn, and Ear), which were cultivated on a bacteriological medium and diagnosed using biochemical and molecular techniques. Results: The results revealed that 376 (71.89%) of the 523 samples had positive culture results, with 240 of those isolates being Staphylococcus (159 isolates, or 30% of the total, were S. aureus, while the remaining 81 isolates, or 16%) were Staphylococcus spp.). MRSA was discovered in 32.1% of the isolates, whereas VRSA was found in 35.4%. After nuc gene identification, 159 (81.95%) of the 194 Staphylococcus included S. aureus. The bacteria had a high resistance to cefotaxime (97.50%), but the lowest resistance to sulfamethoxazole and trimethoprim (30.80%). Conclusion: In the identification of bacteria, the nuc gene-specific primer is more accurate than universal primers and sequencing; nevertheless, the frequency of MRSA and VRSA was high among patients who may develop UTI and nasal carriage when compared to other clinical sources. | ||
| Keywords | ||
| Keywords: Staphylococcus aureus; MRSA; VRSA; nuc gene | ||
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