IDENTIFICATION OF LISTERIA MONOCYTOGENES SEROVAR IN BEEF HOT DOGS | ||||
Egyptian Journal of Agricultural Research | ||||
Article 16, Volume 74, Issue 1, March 1996, Page 161-171 PDF (2.27 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejar.1996.427340 | ||||
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Author | ||||
MAHMOUD Z. MAHMOUD | ||||
Meat and Fish Technology Research Department, Food Technology Research institute, Agricultural Research Centre, Giza, Egypt | ||||
Abstract | ||||
Recent food outbreaks of listeriosis have prompted concenrn about the presence of Listeria monocytogenes in uncooked hot dogs. Therefore 32 samples of hot dogs were collected from different eight shops at Amherst (U.S.A.) through two weeks. Total bacterial counts and Listeria monocytogenes counts were determined in these samples. Thereafter a primary identification for the 24 isolates collected was done to make sure that these isolates belonged to L.monocytogenes. This identificantion included serological identification for L.monocytogenes deteced (22 strains). Analysis for the surface proteins of some of these strains (10 strains) was accomplished by SDS-PAGE. Then the virulence of L.monocytogenes strains was examined. Serological identification and the profiles of tested strains indicated that all the strains belonged to virulent serovar 1/2 b. The virulence test exhibited the same result for all the strains. | ||||
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