Study of the correlation between biofilm formation and antibiotic resistance in Klebsiella Pneumoniae isolated from patients with urinary tract infections | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 14 May 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.375963.2702 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Zahraa Hashim Mohammed  ; Raya Ezat Maroof* ; Estabraq Ali Maklef*
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| College of Health and Medical Techniques, Middle Technical University (MTU), Baghdad, Iraq | ||||
| Abstract | ||||
| Background and aim: Urinary tract infections (UTIs) are the second most common type of bacterial infection worldwide. The second cause of infections is K. pneumoniae. A biofilm is a complex assemblage of microorganisms where the cells are attached to the surface by an extracellular matrix of polymeric slime. This study was designed to show the correlation between antibiotic susceptibility and biofilm production by K. pneumoniae. Methods: A total of 260 urine samples were collected, and one hundred of them (62 females and 38 males) were diagnosed with K. pneumoniae. The urine samples were collected from patients who attended Al-Zahraa Teaching Hospital, Al-Kut Hospital, Fayrouz General Hospital, and Al-Karama Teaching Hospital in Wasit Province, Iraq, during the period from November 2022 to January 2023. The age range of the patients was 9–75 years. The bacterial isolates were identified by biochemical tests and the Vitek2 compact system, while the antibiotic sensitivity was determined by the Kirby-Bauer method, and the biofilm production was estimated by the microtiter plate and tube methods. Results: The results showed that females in the age group (19–28) years were more highly affected than males, with a highly statistically significant difference. There was a correlation between age and sex in patients suffering from UTIs caused by K. pneumoniae. It was shown that K. pneumoniae bacteria were sensitive to imipenem and meropenem in 90% and 85% of isolates, respectively, while they were resistant to ceftriaxone and gentamicin in 81% and 46%, respectively. On the other hand, the results of biofilm production showed that the percentages of non-biofilm producers, weak biofilm producers, moderate producers, and strong producers were 46, 23, 19, and 12%, respectively. There was a significant correlation between antibiotic susceptibility and biofilm production (p ≤ 0.05). Conclusion: Finally, it can be concluded from this study that most bacterial isolates were sensitive to some antibiotics, but the multidrug-resistant isolates were non-biofilm producers. | ||||
| Keywords | ||||
| UTIs; bioflim; antibiotic; CHROM agar; VITEK 2 system | ||||
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