Structural characterization and antiproliferative activity of a symmetrical novel Bi-ethoxy-bi-pyrrole ‘Coelicoflavucin’ from Streptomyces coelicoflavus MFB strains | ||||
| Microbes and Infectious Diseases | ||||
| Articles in Press, Accepted Manuscript, Available Online from 16 May 2025 | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mid.2025.370839.2651 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Ayoub El abbassi   1; ayoub lahmidi2; habti norddine3; Mohammed Menggad1
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| 1Physiopathology and Molecular Genetics, Department of Biology, Faculty of Sciences Ben M’Sik, Hassan II University, Casablanca, Morocco | ||||
| 2Laboratory of Analytical and Molecular Chemistry, Faculty of Sciences Ben M'Sick, Hassan II University of Casablanca, Morocco | ||||
| 3Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Morocco | ||||
| Abstract | ||||
| Background: Bacterial pigments from Streptomyces species like tambjamines and prodiginins are potent cytotoxic compounds against cancer cell lines, and some of them has achieved clinical trials for cancer treatment. The discovery of new related compounds can inspirate the design of new cancer therapies. Methods: This study reported the purification, structural characterization, antiproliferative and antibacterial effects evaluation of the major sub-purified-fraction FA1 isolated from antiproliferative red-like pigments produced by four Streptomyces ceolicoflavus strains (MFB11, MFB20, MFB23, and MFB24). Petroleum ether fraction FA derived from mycelium ethanol extract was purified through column chromatography. Results: The provided FA1 fraction was subjected to spectral analysis (MS, FTIR, 1D and 2D NMR) which indicated that the compound was symmetrical bi-pyrrole bearing two ethoxy extensions, one in each pyrrole ring. The compound (Coelicoflavucin) had no imine side chain compared to known bi-pyrrolic compounds like tambjamines. These distinct chemical structural features, have never been reported elsewhere. Furthermore, bioactivity screening against P3X63Ag8, an apoptosis resistant cancer line to chemotherapeutics by Bcl-xl antiapoptotic proteins overexpression, showed that it has good antiproliferative effects (IC50 = 56.33 µM ± 2.1). Antibacterial assays were negatives on the Gram + and Gram - bacteria tests used, suggesting that this natural pigment is not produced for the defense against bacterial competitors. Conclusions: The newly characterized Coelicoflavucin could provide an advantage with anticancer therapies in cases of resistance to apoptosis, or other pharmacological uses. | ||||
| Keywords | ||||
| Streptomyces ceolicoflavus; natural compounds; Coelicoflavucin anticancer; pigments | ||||
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