Effect of subculture number on pineapple in vitro multiplication and somaclonal variation | ||||
| Arab Journal of Biotechnology | ||||
| Article 3, Volume 17, Issue 2, December 2014, Page 133-142 PDF (14.67 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2014.428032 | ||||
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| Abstract | ||||
| This research was conducted to study the effect of subculture number on multiplication rate, and occurrence of somaclonal variation in pineapple plants (Ananas comosus). Repeating subcultures decreased the multiplication rate in subcultures 1 and 2 (20 times and 9 times, respectively) as it was 6 times in all next subcultures. Repeating subcultures over 5 subcultures decreased the normal plant percentage, it was 95%, 85%, 25% and 8% for subcultures 6, 7, 8 and 9, respectively, and also decreased surviving percentage to reach 85%, 62%, 43% and 21% for subcultures 6, 7, 8 and 9, respectively. SDS protein analysis dendrogram showed two subgroups, where first subgroup included subcultures 2, 3, 4 and 5 subcultures and the second subgroup included subcultures 6, 7, 8 and 9. This subgroup contained a category of subcultures 8 and 9 which were closely related together, whereas subculture 7 was further than this irrelativeness. Subculture 6 resulted in the highest number of shoots with best uniformity (95%) and surviving rate (85%). | ||||
| Keywords | ||||
| Pineapple; Somaclonal variation; Subcultures; Multiplication | ||||
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