Differences in chilling tolerance among genotypes of sweetpotato (Ipomoea batatas (L.) Lam.) | ||||
| Arab Journal of Biotechnology | ||||
| Article 5, Volume 17, Issue 2, December 2014, Page 155-165 PDF (68.17 K) | ||||
| DOI: 10.21608/arjb.2014.428035 | ||||
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| Abstract | ||||
| We aimed to develop a simple method to identify the most chilling tolerant sweetpotato genotypes. Plants at the stage of five to eight most fully expended leaves were used in this experiment. Temperature was reduced in five sequent 144 hrs lasting steps (16/14), (14/12), (12/10), (10/8) and (8/6) °C. Structural changes, chlorophyll fluorescence parameter and content were utilized as selection criteria. The two more tolerant genotypes with orange flesh Abees and Beauregard indicated the overall picture of only minor morphological changes (<11%) in leaves and stem. They showed beside significant lower development of leaf necrosis, generally higher chlorophyll concentrations, a lower reduction of the electron transport rate (ETR) and much less photoinhibition. The protocol employed in this work, with a relative short hardening time, makes it possible to achieve rapid results with good reliability and to handle a large number of genotypes as required in breeding programs. Although the sweetpotato genotypes Beauregard and Abees, selected in this study, might be suitable for growth at low temperatures, even more practical cultivars could be developed by using the method described in this paper. | ||||
| Keywords | ||||
| Chilling tolerance - Chlorophyll fluorescence; Photosynthesis; Sweetpotato | ||||
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