In Silico comparative analysis of type III secretion system effector proteins of Pseudomonas aeruginosa as agents of Acanthamoeba spp. killing
Sumperos, C., Zalzos, J., Ubalde, E., Trinidad, M., Ubiña, A., Valentin, J., Tuguinay, R., Lirio, M. (2025). In Silico comparative analysis of type III secretion system effector proteins of Pseudomonas aeruginosa as agents of Acanthamoeba spp. killing. EKB Journal Management System, 10(2), 63-74. doi: 10.21608/mb.2025.370215.1283
Charlemagne G. Sumperos; Julia Aliyah C. Zalzos; Ed Lois O. Ubalde; Marielle M. Trinidad; An Gheline R. Ubiña; Josiah T. Valentin; Revielmer Alfred A. Tuguinay; Mary Rose F. Lirio. "In Silico comparative analysis of type III secretion system effector proteins of Pseudomonas aeruginosa as agents of Acanthamoeba spp. killing". EKB Journal Management System, 10, 2, 2025, 63-74. doi: 10.21608/mb.2025.370215.1283
Sumperos, C., Zalzos, J., Ubalde, E., Trinidad, M., Ubiña, A., Valentin, J., Tuguinay, R., Lirio, M. (2025). 'In Silico comparative analysis of type III secretion system effector proteins of Pseudomonas aeruginosa as agents of Acanthamoeba spp. killing', EKB Journal Management System, 10(2), pp. 63-74. doi: 10.21608/mb.2025.370215.1283
Sumperos, C., Zalzos, J., Ubalde, E., Trinidad, M., Ubiña, A., Valentin, J., Tuguinay, R., Lirio, M. In Silico comparative analysis of type III secretion system effector proteins of Pseudomonas aeruginosa as agents of Acanthamoeba spp. killing. EKB Journal Management System, 2025; 10(2): 63-74. doi: 10.21608/mb.2025.370215.1283

In Silico comparative analysis of type III secretion system effector proteins of Pseudomonas aeruginosa as agents of Acanthamoeba spp. killing

Microbial Biosystems
Article 7, Volume 10, Issue 2, June 2025, Page 63-74  XML PDF (1.12 MB)
Document Type: Original Article
DOI: 10.21608/mb.2025.370215.1283
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Authors
Charlemagne G. Sumperos; Julia Aliyah C. Zalzos; Ed Lois O. Ubalde; Marielle M. Trinidad; An Gheline R. Ubiña; Josiah T. Valentin; Revielmer Alfred A. Tuguinay; Mary Rose F. Lirio email orcid
Department of Medical Technology, Institute of Health Sciences and Nursing, Far Eastern University – Manila, Nicanor Reyes St., Sampaloc, Manila, Metro Manila, Philippines.
Abstract
The Type III Secretion System (T3SS) of Pseudomonas aeruginosa plays a critical role in its pathogenicity by delivering effector proteins directly into host cells. This study conducted an in silico comparative analysis of four T3SS effector proteins (ExoS, ExoT, ExoU, and ExoY) to elucidate their molecular mechanisms in targeting Acanthamoeba spp., a genus of free-living amoebae with significant public health implications. Using bioinformatic tools, we examined the molecular structures, physicochemical properties, and cytotoxic mechanisms of these effectors. Structural characterization revealed distinct functional domains: ExoS and ExoT share overlapping activities but differ in their mechanisms, with ExoS disrupting cellular processes via ADP-ribosyltransferase activity and ExoT impairing phagocytosis through its GTPase-activating protein domain. ExoU’s phospholipase activity leads to membrane disruption and necrotic cell death, while ExoY interferes with cytoskeletal integrity through adenylate cyclase activity, increasing intracellular cAMP levels. Molecular docking analyses demonstrated stable binding interactions between these effectors and Acanthamoeba castellanii, highlighting their specific cytotoxic pathways. These findings provided insights into the pathogenesis of P. aeruginosa and offer a foundation for the development of novel therapeutic strategies against Acanthamoeba infections.
Keywords
Amoebae; host-pathogen interaction; molecular docking; phospholipase
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