INNOVATIVE IDEAS FOR RAM SEMEN FREEZING a. EFFECT OF EXTENDER COMPOSITION, ADDITION METHOD OF EXTENDER AND TEMPERATURE AT ADDITION EXTENDER ON RAM SEMEN FREEZABILITY | ||||
| Menoufia Journal of Animal Poultry and Fish Production | ||||
| Article 9, Volume 9, Issue 4, April 2025, Page 45-56 PDF (535.42 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjapfp.2025.376121.1028 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mamdouh Abbas Elghonamy  1; , H. Abd El Rahman2; I.M. Seddik2; abdallah mohamed3
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| 1teacher | ||||
| 2Animal Production Department, Faculty of Agriculture, Minufia Universit, Egypt. | ||||
| 3قسم الانتاج الحيوانى كلية الزراعة جامعة المنوفية | ||||
| Abstract | ||||
| Semen was collected from four adult Ossimi rams using an artificial vagina to study the effects of the extender addition either on one-step or two-step technique, with three temperatures at extender addition to raw semen at (25°C, 15°C and 5°C) on ram semen freezability in liquid Nitrogen (-196°C). Two new extenders were used, Tris-based extender (A-extender) and Tris-based extender containing 35% skim milk (B-extender). The final glycerol level was 14.5% in two extenders, and the dilution rate was 1:6 (v/v). The equilibration period was 3 hours. The frozen semen was thawed at 42°C for 35 seconds. Sperm motility and livability were determined to evaluate semen at various freezing and thawing stages. A-extender yielded the best post-thaw motility and livability (44.4 and 43.8 %, respectively) compared with B-extender, which recorded 33.1 and 32.7% values for motility and livability, respectively. One-step method yielded better values (P<0.01) of post-thaw motility and livability as 40.4 and 40.0 %, respectively, compared with the two-step method, which revealed 37.2 and 36.5% values, respectively. Addition of extender at 15°C yielded better results (P<0.01) of motility and livability than addition at 25°C and significantly better than 5°C. It could be advisable to use A-extender added to raw ram semen in one step at 15°C to improve semen freezability. | ||||
| Keywords | ||||
| : Ram semen; Freezing; Extender; Glycerol; Addition method | ||||
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