Identification of differentially expressed drought-responsive transcripts in Egyptian cotton | ||||
| Arab Journal of Biotechnology | ||||
| Article 5, Volume 18, Issue 1, June 2015, Page 55-66 PDF (437.37 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2015.428413 | ||||
 View on SCiNiTO | ||||
| Abstract | ||||
| There is no enough information available on drought-modulated gene(s), which can be a valuable gene pool for improving modern cotton cultivars. The major objective of current study was to identify the responsive transcripts in two elite Egyptian cotton varieties (Giza 88 and Giza 90) under drought stress. To accomplish this, 17% PEG6000 drought stress was applied to seedlings of both cultivars under greenhouse conditions. RNA isolation, differential display (DD) PCR, silver staining gels, purification of DD fragments, and sequencing approaches were applied. A total of 13 out of 27 DD fragments were sequenced and 12 fragments were found with high homology to drought responsive gene families such as peroxidase, oxidoreductase, and kinases. Our study is considered as an addition to the global GenBank database of functional drought responsive transcripts in Egyptian cotton. Moreover, the 12 identified transcripts in our study can be used in multiple bioinformatics and transcriptomics cotton research. | ||||
| Keywords | ||||
| Drought stress; DD- PCR; Gossypium barbadense; cDNA Transcripts; GenBank Database | ||||
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