Molecular identification and characterization of some Egyptian marine cyanobacteria | ||||
| Arab Journal of Biotechnology | ||||
| Volume 18, Issue 2, December 2015, Page 113-134 PDF (16.93 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2015.428421 | ||||
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| Abstract | ||||
| Molecular techniques were applied to study three cyanobacterial isolates (Spirulina, Oscillatoria and Anabaena). Genetic diversity among strains was tested by PCR amplification and DNA sequencing of 16S rDNA gene using universal primers and the obtained sequences wase compared with those available in the GeneBank Database. Phylogenetic tree was inferred by NJ distance method. For more exact distinguishing proof, a set of oligonucleotide primers were developed for the specific amplification of 16S rDNA gene from the three cyanobacterial isolates by PCR. Both universal primer-based sequencing and specific PCR amplification of 16S rDNA gene revealed the same results as the three cyanobacterial species were identified as: Spirulina sp. EEW5, Oscillatoria accuminata PCC 6304 and Anabaena variabilis RPAN54, revealing the usefulness of such PCR fingerprinting profiles for the identification of cyanobacterial isolates. The chemical analysis of cyanobacterial isolates showed that Spirulina had the highest protein percentage and amino acids content in addition to considerable amounts of phytohormones. Oscillatoria recorded higher content of photosynthetic pigments including phycobiliproteins. | ||||
| Keywords | ||||
| Cyanobacteria; PCR; 16S rDNA; Spirulina; Oscillatoria; Anabaena; Chemical analysis | ||||
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