Identification and evaluation of phytochemicals extracted from Syzygium aromaticum (L.) and Cinnamomum zeylanicum (L.) on some antibiotic-resistant bacteria | ||
| Microbial Biosystems | ||
| Article 12, Volume 10, Issue 2, June 2025, Pages 106-116 PDF (727.91 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/mb.2025.315210.1160 | ||
| Authors | ||
| Khalid Abed Yasseen* ; Kareem Talib Khshan | ||
| Department of Biology, Faculty of Sciences , Kufa University, Iraq. | ||
| Abstract | ||
| The experiment involved two plants, Syzygium aromaticum and Cinnamomum zeylanicum, which were used to extract plant parts using 96% ethanol solvent and distilled water for each method. Crude extracts at concentrations of 1000 mg/ml, 500 mg/ml and 100 mg/ml were prepared for the detection of antibacterial activity. Two types of extract are used for treated bacteria studies for inhibition zones. The results of chemical tests for the ethanol and aqueous extracts of the two plants showed positive results for phytochemicals. This effect was shown by the peaks identified by GC-Mass. We determined the antibacterial activity of plant extracts as a criterion for evaluating growth inhibition. The crude extracts were tested against two types of bacteria, which were Gram positive and Gram negative including Staphylococcus aureus and Escherichia coli, which cause urinary tract infections. The results indicated that the plant extracts with high amounts of phytochemicals are rich sources of antibacterial properties that cause urinary system diseases. All concentrations of extracts inhibited the growth of bacteria, and the effect increased with increasing concentration. There are significant differences between concentration 1000 µg/ml and other concentrations according to the inhibition zone; also, there are significant differences between the effect of all plants on bacteria. | ||
| Keywords | ||
| Aqueous extract; GC-MS (Gas Chromatography–Mass Spectrometry); inhibition zone; Staphylococcus aureus; urinary tract infection | ||
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