Influence of PAMP-induced systemic resistance in potato suspension cells and seedlings on defense signaling markers and infection with Phytophthora infestans | ||
| Arab Journal of Biotechnology | ||
| Volume 19, Issue 1, June 2016, Pages 35-50 PDF (868.04 K) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/arjb.2016.428488 | ||
| Abstract | ||
| Colonization of roots by selected strains of fluorescent Pseudomonas spp. or their elicitors can trigger induced systemic resistance (ISR) against foliar pathogens in a plant species-specific manner. It has been suggested that early responses in cell suspension cultures in reply to rhizobacterial elicitors, such as generation of active oxygen species (AOS) and extracellular medium alkalinization (MA), are linked to the development of ISR in whole plants. In the present study, the pathogen-associated molecular patterns (PAMPs) i.e. lipopolysaccharides (LPS), siderophores, and the flagellin were tested for their effects on the induction of ISR in either potato cell suspension or seedlings. LPS among all PAMPs provoked an almost immediate burst in potato cell suspensions (H2O2 24.8 nmoles /g f. w. after 20 min). Filagellin showed a slower and weaker induction of AOS effect compared to LPS, whereas Siderophores did not show such induction. Siderophores were almost as active as LPS in induction of extracellular medium alkalinization, whereas flagellin was approximately half-so. In contrast to the refractoriness of the potato cells to re-stimulation of an oxidative burst with PAMPs, the cells here were able to almost fully re alkalinize the medium upon repeated stimulation. All studied PAMPs were able to generate cell death in potato cell suspension to different levels. Northern blotting of the defense marker genes PAL, GST and ACO showed a remarkable induction at 3-9 hours post inoculation with the PAMPs and the induction effect subsided at 12-24 h. Potato suspension cells induced by Siderophores completely inhibited sporangia germination, whereas those induced by LPS led to deterioration of the sporangia. Elicitation of potato seedlings with all studied PAMPs resulted in significant reduction of disease index, AUDPC. Obtained significant correlation between ISR induction in potato cell suspension and seedlings suggested the potential to explore the response of potato plant to new elicitors with cell suspension first as it is a time and effort saving technique. | ||
| Keywords | ||
| Potato; Phytophthora infestans; ISR; Cell culture; Resistance genes | ||
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