Plant regeneration in date palm (Phoenix dactylifera) cv. Khalas overcoming four different fungus contaminations by using biological control | ||||
| Arab Journal of Biotechnology | ||||
| Volume 19, Issue 2, December 2016, Page 135-148 PDF (4.49 MB) | ||||
| DOI: 10.21608/arjb.2016.428495 | ||||
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| Abstract | ||||
| In the present investigation, date palm plant regeneration procedure overcoming four different fungus contamination using biological control was described. Three different concentration treatments of actinomycetes culture filtrate (5, 10 and 15%) from two isolates (Streptomyces bobilii and Streptomyces grisiobrunneus) were used to study their effect in overcoming four different fungi which contaminate in vitro date palm i.e. Fusarium moniliforme, Rhizoctonia solani, Macrophomina phaseolina and Alternaria alternata. Medium supplemented with S. bobilii surpassed medium supplemented with S. grisiobrunneus in regeneration characteristic values. Actinomycetes 10 % treatment proved to be the best for enhancing both survived explant and callus induction percentage, while 15% treatment proved to be the best for enhancing regenerated shoots per callus. Herein, we report improved regeneration system for Khalas cultivar, which is strongly needed for successful genetic transformation, overcoming fungi contamination destructiveness. | ||||
| Keywords | ||||
| Date palm; Khalas; Regeneration; Contamination; Biological control | ||||
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