Developing of QTL Associated with Salinity Tolerance in Chickpeas | ||||
| Arab Journal of Biotechnology | ||||
| Volume 19, Issue 2, December 2016, Page 149-162 PDF (460.88 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2016.428496 | ||||
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| Abstract | ||||
| Soil salinity is one of the major abiotic stress which severely limits agricultural crop production throughout the world, and is increasing particularly in the irrigated agricultural areas as is the case in Egypt. Approximately, 33% of the irrigated land in Egypt is salt affected. Chickpea (Cicer arietinum L.) is an important grain legume that plays a significant role in the nutrition of the poor in the developing world. In this research, we used rich chickpea diversity panel collected from the GeneBank of the International Center for Agricultural Research in the Dry Area (ICARDA). This collection was selected by using the focused identification of germplasm strategy (FIGS). DNA of this core collection was extracted for molecular characterization using 16 SSR and 3 ISSR polymorphic markers. Association mapping was conducted by using Single-Locus-F test. The results indicated significant differences between the chickpea genotypes. Based on the average between the two locations, seven tolerant genotypes IGs (70275, 70249, 70351, 70782, 8447, 70330, 9434 and 70309) have been identified as tolerant genotypes. Association mapping indicated that SSR marker TAA170 was associated with salinity tolerance (P = 0.006201848). The results indicated that SSR marker TAA170 is close to the acidic endochitinase-like protein gene which is thought to be responsible for the salinity stress tolerance. This marker could be a candidate for marker assisted selection in chickpea. | ||||
| Keywords | ||||
| Association mapping; Chickpea diversity; QTL; Salinity tolerance; Acidic endochitinase like protein gene | ||||
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