Production of recombinant human interferon gamma by batch fermentation in E. coli system | ||||
| Arab Journal of Biotechnology | ||||
| Volume 20, Issue 1, June 2017, Page 21-32 PDF (1.92 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2017.428498 | ||||
 View on SCiNiTO | ||||
| Abstract | ||||
| To improve the expression of recombinant human interferon gamma (rhIFN-γ) protein in recombinant E. coli Rosetta DE3 bacterial cells, the effect of different factors for the over production of rhIFN-γ, including type of culture media, pH and type of inducer, has been evaluated in experimental shaking flasks. A simple batch fermentation process was carried out and the growth rate of recombinant E. coli Rosetta DE3 has been monitored using optical density measurements and wet cell weight. The LB medium was used and the dissolved oxygen level was maintained at 30 40% of air saturation, by control of the inlet air and agitation rate. The pH of media was sustained at pH 7. The inducer was lactose at 2mM final concentration, added after 3 h fermentation, and the stationary phase of growth started at 9 h of batch process. The final cell weight of the batch fermentation was 7g/l media after 12 h of starting process. The immunogenicity of rhIFN-γ protein has been tested against the rabbit polyclonal human interferon antibodies by western blot analysis. Based on these small-scale experiments, optimum operating conditions were chosen to scale up a better pilot process production of rhIFN-γ protein. | ||||
| Keywords | ||||
| Recombinant human interferon gamma; Batch fermentation- Lactose inducer - E. coli Rosetta DE3. INTRODUCTIO | ||||
|
Statistics Article View: 50 PDF Download: 24 |
||||
