Molecular differentiation for six genotyps of sugarcane (Saccharum spp.) using scot marker | ||||
Arab Journal of Biotechnology | ||||
Volume 20, Issue 1, June 2017, Page 63-70 PDF (1.11 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/arjb.2017.428501 | ||||
![]() | ||||
Abstract | ||||
Start codon targeted polymorphism (SCoT), a novel molecular marker targeting conserved region surrounding start codon proved its efficiency in genetic diversity analysis and fingerprinting. In this study, 151 amplified fragments were obtained by using 10 SCoT primers with 6 genotypes of sugarcane (Saccharum spp.), of which 98 (64.9%) were polymorphic. And, there were 44 unique fragments divided into positive and negative markers related to important characters in sugarcane species. Highest percentage of similarity (87.32%) was found between genotype 5 (G. 54-9) and genotype 6 (BOT 41) utilizing NTSYSpc 2.01b software through UPGMA to find the genetic relationship illustrated in phylogenetic tree. | ||||
Keywords | ||||
Genetic diversity; Sugarcane; SCoT molecular markers; Saccharum spp | ||||
Statistics Article View: 44 PDF Download: 23 |
||||