Effect of different conservation periods with different sucrose concentrations on conserving somatic embryo clusters of date palm (Phoenix dactylifera L.) under minimal growth | ||||
| Arab Journal of Biotechnology | ||||
| Volume 20, Issue 2, December 2017, Page 105-122 PDF (2.86 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2017.428790 | ||||
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| Abstract | ||||
| The present study was conducted to investigate the possibility of using in vitro slow growth storage for date palm (Phoenix dactylifera L.) germplasm conservation to promote germplasm exchange and rapid propagation when necessary. Multiplied somatic embryo clusters of date palm cv. Sukkary were used as the conserved explants. Different conservation periods (4, 8, and 12 months) with different sucrose concentrations (30, 60, 90 or 120 g/l) supplemented to conservation medium which consists of 1/2 strength Murashige and Skoog (MS) basal salts medium, 30 g/l mannitol, 0.05 mg/l (BA) benzyladenin, 0.1 mg/l (NAA) naphthalene acetic acid and 8 g/l agar, under low temperature (15 C) and dark incubation conditions, were examined to determine the survival and re-growth capacity of conserved explants after returning to the normal growth conditions. Contents of total soluble sugars (TSS), non reducing sugars (NRS) and reducing sugars (RS) were also determined as physiological changes during conservation periods. Results showed that conservation medium supplemented with 90 or 120 g/l sucrose gave the highest significant value of survival percentage, after 12 month conservation period. Best recovery performance under normal growth conditions for conserved somatic embryo clusters under studied minimal growth conditions was achieved when 90 g/l sucrose was used in conservation medium for 8 months | ||||
| Keywords | ||||
| Germplasm conservation; In vitro; Sucrose; Low temperature; Phoenix dactylifera L; Slow growth storage | ||||
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