Determination of genetic diversity using SDS-PAGE and RAPD markers and active compound gene in Zygophyllaceae Genotypes | ||||
| Arab Journal of Biotechnology | ||||
| Volume 20, Issue 2, December 2017, Page 123-138 PDF (1.74 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2017.428791 | ||||
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| Abstract | ||||
| Biochemical variability was conducted by protein electrophoretic patterns among ten genotypes of Zygophyllaceae. RAPD molecular markers were used to estimate the polymorphism among these genotypes. SDS-PAGE revealed 36 total bands (67% polymorphism). Five RAPD primers revealed 80 bands with the highest polymorphism (95%). Based on similarity matrix of overall analysis (SDS-PAGE and RAPD), the dendrogram was constructed and separated the ten Zygophyllaceae genotypes into two main clusters. The first included the Zygophyllum coccineum genotypes from two locations, Z. simplex and Nitraria retusa from three locations, the second included Fagonia indica from two locations, Peganum harmal and Tribulus terrestris. The molecular marker was better than biochemical marker to detect genetic diversity among Zygophyllaceae genotypes. Results showed that the length of the TDC (tryptophan decarboxylase) gene for alkaloid production at the fragment of 1770 bp. BLAST sequence alignment and phylogenetic tree analyses revealed that the TDC fragment is genetically close to Rauvolfia verticillata and Catharanthus roseus. This study can be used in the future for conservation, breeding and improvement programs | ||||
| Keywords | ||||
| Zygophyllaceae; Isozymes; ISSR; TDC gene; sequence alignment and phylogenetic tree | ||||
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