In vitro fertilization and embryonic development of post thawed buffalo oocytes vitrified with soybean lecithin | ||||
| Arab Journal of Biotechnology | ||||
| Volume 21, Issue 1, June 2018, Page 1-10 PDF (772.87 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2018.428792 | ||||
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| Abstract | ||||
| Current study was carried out to the evaluate the impact of addition of zero (control), 1, 2.5, 5, 10% soybean lecithin to the vitrification solution media and its subsequent in vitro fertilization and embryo development of post-thawed immature and matured buffalo oocytes cryopreserved with stepwise vitrification. Although the addition of lecithin to the vitrification solution did not significantly affect in vitro fertilization(IVF) of both immature and mature oocytes, lecithin increased penetration and fertilization rates of immature (50.0 and 33.3%) and mature oocytes (52.6 and 36.8%) at the level of 5 and 2.5% respectively. Trials of embryo In Vitro culture(IVC) revealed that 2.5% addition of lecithin significantly improved (p<0.05) both blastocyst formation and total embryonic development (9.5 and 28.6% resp.) of vitrified-thawed mature oocyte than control ones after 7 days of culture. Similar insignificant trend was observed for vitrified-thawed immature oocytes at the level of 5% lecithin. Our results revealed that, soybean lecithin addition to vitrification solution might have a protective potential on vitrified buffalo oocytes after warming and their subsequent embryo development in vitro. | ||||
| Keywords | ||||
| lecithin; buffalo oocytes; vitrification; IVF; IVC | ||||
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