Possible implication of melatonin receptor 1A and Arylalkylamine N acetyltransferase genes polymorphisms for seasonal reproduction in Egyptian sheep breeds | ||||
| Arab Journal of Biotechnology | ||||
| Volume 21, Issue 1, June 2018, Page 11-26 PDF (1.88 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2018.428793 | ||||
 View on SCiNiTO | ||||
| Abstract | ||||
| This study was carried out for detection of polymorphisms in melatonin receptor 1A (MTNR1A) and Arylalkylamine N acetyltransferase (AA-NAT) genes and their association with reproductive traits. Blood samples of 126 Animal from three Egyptian sheep breeds were collected. DNA was extracted and subjected to PCR-RFLP using RsaI and SmaI enzymes. Two alleles (C and T), three genotypes (CC, CT and TT) for MTNR1A gene also (A and G), (GG, GA, and AA) for AA NAT gene were detected. Alleles C and A, genotypes CT and GA showed the highest frequency for MTNR1A and AA-NAT genes respectively. Association analysis of MTNR1A SNP revealed a significant association in Ossimi and Rhmani breeds with age of first lambing and C allele seems to be the favorable allele. Results for AA-NAT SNP demonstrate significant differences in Ossimi with age of first lambing and litter size and in Rhmani with lambing interval and G allele seems to be the desirable allele. Concerning to first conception season the study revealed that ewes carrying CT exhibited significantly lower age of first lambing in unfavorable season. Also, GG ewes exhibited significantly lower age of first lambing in early favorable followed by unfavorable seasons. According to personal knowledge this is the first study concerned with this association in Egyptian sheep breed. In conclusion: the polymorphisms achieved in this study could be measured as genetic markers suitable for improving reproductive efficiency during unfavorable season and the obtained desirable genotypes could be taken into account in new genetic selective schemes. | ||||
| Keywords | ||||
| MTNR1A; AA-NAT; Egyptian sheep; polymorphism; Reproductive seasonality; PCR RFLP | ||||
|
Statistics Article View: 48 PDF Download: 21 |
||||
