Biochemical analysis of glucose level and superoxide dismutase activity in buffalo follicular fluid and blood Serum | ||||
| Arab Journal of Biotechnology | ||||
| Volume 21, Issue 1, June 2018, Page 27-34 PDF (920.13 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2018.428794 | ||||
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| Abstract | ||||
| The reducing reproductive efficiency is one of the critical problems of the water buffalo, so the recent studies have indicated alterations in the follicular fluid and blood serum composition during such condition. The present study, therefore, aims at assaying the changes of follicular fluid glucose level and superoxide dismutase (SOD) activity in different size follicles and assaying these parameters in follicular fluid and blood serum during different phases of estrous cycle in the buffalo. Blood samples and ovaries were collected from 20 cyclic buffaloes of known estrous phase (follicular or luteal). Follicular fluid was aspirated from small sized ovarian follicles (2-4 mm) and medium sized follicles (5-8 mm). Glucose level and SOD activity were significantly higher (P < 0.05) in medium sized follicles (85.89 ± 1.59 mg/dl and 53.66 ± 0.76U/ml, respectively) than in small sized follicles (72.09 ± 0.84 mg/dl and 49.30 ± 0.41U/ml, respectively). Moreover, both the follicular fluid glucose level and SOD activity were significantly higher (P < 0.05) during the follicular phase than the luteal phase either in medium or small follicles. Also, the blood serum SOD activity was higher (P < 0.05) during the follicular phase (45.47 ± 0.50 U/ml) than in the luteal phase (38.46 ± 0.43 U/ml). In conclusion, Glucose level and SOD activity were significantly high in medium follicles follicular fluid and in serum during follicular phase of estrous cycle in buffalo. | ||||
| Keywords | ||||
| Buffalo; follicular fluid; glucose; SOD activity | ||||
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