A genetic linkage map of the chickpea, Cicer arietinum, based on microsatellite markers and localization of the Fusarium vascular wilt resistance locus | ||||
| Arab Journal of Biotechnology | ||||
| Volume 21, Issue 1, June 2018, Page 59-74 PDF (1.25 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2018.428796 | ||||
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| Abstract | ||||
| Fusarium wilt, caused by Fusarium oxysporum f.sp. ciceri (Foc), is one of the tremendous biotic stresses instigating huge yield losses in chickpea (Cicer arietinum). A total of 853 microsatellites were screened in two chickpea parents (FLIlP97-7 and ILC482). One parent, FLIP97-7, is Fusarium wilt resistant to the fungus Fusarium oxysporum, while the other parent, ILC482, is susceptible. One hundred forty four recombinant inbred lines, originated from a cross between FLIP97-7 and ILC482, segregating for this character were mapped using SSR markers. The study detected variation between the two parents, where twenty-six Simple sequence repeats (SSRs) were polymorphic. The SSR microsatellites were mapped on the 144 recombinant inbred lines segregating for this character. Linkage analysis showed that the Fusarium vascular wilt resistance locus race 0 (Foc-0) was located on linkage group (1) around 19.7 centi Morgan away from the NCPGR77 microsatellite marker. This study may help in improving the chickpea breeding programs through the development of diagnostic markers that could contribute significantly in Marker Assisted Selection. | ||||
| Keywords | ||||
| Fusarium oxysporum ciceris; Recombinant Inbred Lines (RILs); Molecular markers; SSR; Linkage analysis | ||||
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