Molecular and Biochemical Genetic Studies of Some Mutants in Drosophila melanogaster | ||||
| Arab Journal of Biotechnology | ||||
| Volume 21, Issue 2, December 2018, Page 75-92 PDF (1.71 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2018.428797 | ||||
 View on SCiNiTO | ||||
| Abstract | ||||
| Ten mutant lines which have been included single, double and triple mutants (four stocks were built up) in addition to the wild type of Drosophila melanogaster were used to study the genetic fingerprint in nuclear DNA on the molecular and biochemical levels. The results of molecular analysis in nuclear DNA by using random amplified polymorphic DNA revealed polymorphic ratio ranged from 73.33% to 100% with eight unique bands and total fragments of 63 with an average number of 12.6 fragments /primer. The genetic similarity of nuclear DNA ranged from 44.8%(dp ,y ) to 82.5%(dp,OR) for (RAPD-PCR) and the cluster analysis based on similarity matrices separated the single mutant lines from the double and triple mutant lines except white dumpy ebony. Also, the single mutant lines were separated to subgroup according to the chromosome which carries the mutant. On other hand, the biochemical analysis revealed that the different mutational load didn't affect protein concentration in different mutant lines, while some mutant lines show significant excess of amino acid polymorphism.These results led to the possibility of using molecular and biochemical DNA as a reliable and quick method for studying the genetic fingerprint in these mutant lines and other organisms. | ||||
| Keywords | ||||
| Drosophila melanogaster; RAPD; fingerprinting; molecular markers; biochemical markers | ||||
|
Statistics Article View: 44 PDF Download: 17 |
||||
