Determination of Suitable Gamma-Ray Dose for Mutation Induction in Garlic (Allium sativum L.) and Identification of Genetic Diversity by RAPD and ISSR markers
(2019). Determination of Suitable Gamma-Ray Dose for Mutation Induction in Garlic (Allium sativum L.) and Identification of Genetic Diversity by RAPD and ISSR markers. EKB Journal Management System, 22(1), 25-42. doi: 10.21608/arjb.2019.428805
. "Determination of Suitable Gamma-Ray Dose for Mutation Induction in Garlic (Allium sativum L.) and Identification of Genetic Diversity by RAPD and ISSR markers". EKB Journal Management System, 22, 1, 2019, 25-42. doi: 10.21608/arjb.2019.428805
(2019). 'Determination of Suitable Gamma-Ray Dose for Mutation Induction in Garlic (Allium sativum L.) and Identification of Genetic Diversity by RAPD and ISSR markers', EKB Journal Management System, 22(1), pp. 25-42. doi: 10.21608/arjb.2019.428805
Determination of Suitable Gamma-Ray Dose for Mutation Induction in Garlic (Allium sativum L.) and Identification of Genetic Diversity by RAPD and ISSR markers. EKB Journal Management System, 2019; 22(1): 25-42. doi: 10.21608/arjb.2019.428805

Determination of Suitable Gamma-Ray Dose for Mutation Induction in Garlic (Allium sativum L.) and Identification of Genetic Diversity by RAPD and ISSR markers

Arab Journal of Biotechnology
Volume 22, Issue 1, June 2019, Page 25-42  XML PDF (1.47 MB)
Document Type: Original Article
DOI: 10.21608/arjb.2019.428805
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Abstract
As an initial step in a mutation breeding program to improve two Egyptian garlic (Allium 
sativum L.) clonal cvs Balady and Sids-40, this experiment was conducted to test the radio
sensitivity for these clones to different gamma-ray doses (0, 2, 4, 6, and 8 Gy) and to evaluate the 
genetic diversity between different doses treatments by using the molecular markers. The suitable 
dose that induce mutation without adverse effect on germination and plant growth was determined 
using 50% plant mortality (LD50) based on the probit analysis. RAPD and ISSR markers were used 
to evaluate the genetic diversity. Increasing gamma-ray dose led to a decrease in the germination 
percentage and an increase in the mortality percentage. The inhibitory effects of high doses (6 and 
8 Gy) and the stimulant effects of low doses (2 and 4 Gy) were clearly shown on the plant growth 
and the formed bulb traits. The LD50 was 6.42 Gy for 'Balady' and 6.93 Gy for 'Sids-40'. ISSR 
markers revealed a higher polymorphism (100 %) than did RAPD (68.6 %). The genetic similarity 
coefficient ranged from 0.5812 to 0.87591. Moreover, the constructed dendrogram classified Sids
40 and Balady with 6 Gy into one cluster and all the other treatments and Balady were grouped 
into another cluster, thus indicating higher genetic distinctness among different treatments of 
gamma rays. Accordingly, this study suggested the possibility of using the probit analysis to 
determine the appropriate dose of gamma irradiation, as well as using ISSR and RAPD markers to 
determine the genetic diversity in garlic.  
Keywords
Allium sativum; LD50; Probit analysis; RAPD; ISSR
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