Genetic diversity among some broomrape (Orobanche) species from Egypt using SRAP and ISSR markers | ||||
| Arab Journal of Biotechnology | ||||
| Volume 22, Issue 1, June 2019, Page 63-78 PDF (1.64 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2019.428807 | ||||
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| Abstract | ||||
| Broomrape, a noxious parasitic weed from several families, causes severe damage to economically important vegetables and field crops in Egypt. It can reduce crop yield as much as 90 to 100%. Identification of broomrape species based on morphological characters faces major difficulties. Therefore, the precise discrimination of such parasitic weed is considered the first step toward effective and specific weed management. Thus, in this study molecular markers were used as powerful and rapid identification techniques for the most major Orobanche species in Egypt. Sixteen sequence related amplified polymorphism (SRAP) primers and nine inter simple sequence repeat (ISSR) primers were employed to assess the genetic diversity among three most important Orobanche species. The SRAP and ISSR analyses showed that, 132 out of 170 and 80 out of 106 markers, respectively, were detected as polymorphic markers (77.6 % and 75.4 %) among the tested Orobanche species. The Orobanche species were characterized by 32 genotype-specific markers, 20 for SRAP and 12 for ISSR that would be considered as useful markers for Orobanche species. UPGMA cluster analysis separated the three broomrape species into two main clusters, the first one comprised of two species O. ramosa which infect different plants and O. aegyptiaca. While, the second cluster included only O. crenata. This clustering indicates that the tested species have common genomic segments. Our results indicated that SRAP–based molecular could be used for characterization of the studied broomrape species as considered more reliable and robust than other molecular markers used. Moreover, investigating the variability in broomrape species is crucial when attempting to develop weed control means. | ||||
| Keywords | ||||
| Broomrapes; Orobanche spp; Genetic diversity; ISSR markers; SRAP markers | ||||
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