Microsatellite markers associated with salt tolerance in Sorghum bicolor L. | ||
| Arab Journal of Biotechnology | ||
| Volume 22, Issue 2, December 2019, Pages 93-102 PDF (1.91 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/arjb.2019.428809 | ||
| Abstract | ||
| In this study, the genetic diversification and the salinity tolerance potential of six sorghum cultivars was screened by microsatellite markers (SSR) related to salt tolerant genes. The data indicated that, the PIC (Polymorphic information content) value varies from 0 to 1 with a mean of 0.6. The SSR primers Umc1862, Umc1501 and Umc1545 showed the highest PIC values 1, 1 and 0.91 respectively, whereas Phi031 and Umc1719 showed 0.33 value. Cluster analysis based on salt tolerant gene based SSR markers, assembled cultivars into two independent clusters as tolerant and susceptible. The first cluster contains Horas and Maka-244 cultivars of highly salt tolerance, where the total scorable alleles of these genotypes was 43 and 41, respectively, while the second cluster contains Giza-420, PM, Special-85 and Special-90 cultivars of salt sensitivity, which registered 31 alleles of total scorable alleles, suggesting the prospect benefit of these SSR-markers in mapping of salinity-related traits. | ||
| Keywords | ||
| Genetic diversification; sorghum; salt tolerance; Microsatellite; SSR markers | ||
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