Morphological and Molecular (ISSR and SCoT) characterization of some grape cultivars in the North Sinai | ||||
| Arab Journal of Biotechnology | ||||
| Volume 22, Issue 2, December 2019, Page 119-134 PDF (1.09 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/arjb.2019.428811 | ||||
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| Abstract | ||||
| The present study was focusing on the morphological characterization of six grape cultivars collected from different locations in North Sinai. Also, to assess the genetic diversity using molecular markers (ISSR and SCoT) among these cultivars and four commercial genotypes. Data analysis of the cluster characters showed that highly significant differences were observed among the six cultivars for the average cluster weight and the number of berries/cluster. While, cluster length, and the average of cluster compactness revealed significant differences. Moreover, the results of berry physical characteristics showed a significant variation among the six cultivars. Nine ISSR and ten SCoT primers were used to determine the level of polymorphism, molecular identification of unique markers, and the estimation of genetic distances for the ten grapevine cultivars (6 cultivated in North Sinai and four commercial cultivars). The ISSR primers amplified 93 amplicons of which 51 were polymorphic. While, SCoT primers showed 136 amplicons, the total number of polymorphic amplicons was 52. The two molecular markers revealed positive unique markers only. The number of positive unique markers was 9 and was useful in identifying 5 genotypes out of the 10 cultivars. The similarity indices ranged from 92% to 76% and 94% to 84% for ISSR and SCoT, respectively. The cluster analysis exhibited a tendency the cultivars collected from North Sinai are groups in the same subcluster except Banaty White from the two molecular markers. It is concluded that we need the additional grapevine germplasm were collected from other locations and more morphological characters study to ensure the genetic diversity in grapevine germplasm. | ||||
| Keywords | ||||
| North Sinai; Grapevine; Morphology; Molecular Markers (ISSR; SCoT) | ||||
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