Assessment of inter- and intra-variations in some olive (Olea europaea) cultivars using molecular markers (ISSR and SCoT) | ||
| Arab Journal of Biotechnology | ||
| Volume 22, Issue 2, December 2019, Pages 135-148 PDF (1.64 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/arjb.2019.428812 | ||
| Abstract | ||
| Genetic variations among and within 15 cultivated olive (Olea europaea L.) genotypes distributed in three farms in El-Arish, Egypt were screened using two PCR-based molecular markers; Inter Simple Sequence Repeat (ISSR) and Start codon targeted (SCoT). Nine ISSR primers and 15 SCoT primers successfully produced 170 (89%) and 347 (91.1%) polymorphic bands. Fourty five and 108 positive unique cultivar-specific bands were generated by ISSR and SCoT markers, respectively. ISSR 1 and ISSR 18 could characterize genotypes within Chemlali population. While, within Manzanillo population, genotypes were differentiated using ISSR 20. On the other hand, SCoT2, SCoT4 and SCoT13 could successfully differentiate genotypes within Picual population. Moreover, Coratina population was identified by SCoT14 and SCoT22 primers. PIC values, Nei’s gene diversity and Shannon index were 0.21, 1.27 and 0.31, respectively for ISSR marker compared to 0.20, 0.17 and 0.29 respectively for SCoT marker. Picual genotypes had the highest values of Na (1.29), Ne (1.17), H (0.10) and I (0.16) and Koroneiki population showed the lowest values for these parameters (1.20, 1.11, 0.07 and 0.11), respectively. Analysis of molecular variance (AMOVA) showed significant genetic variations among (48%) and within (52%) olive populations. Dendrogram obtained by UPGMA clustering confirmed the genetic relationships among as well as between olive cultivars. This study suggests that combining genetic variations data resulting from ISSR and SCoT have a higher potentiality in assessing intra- and inter- varietal variations in olive genotypes supporting continued progress in olive breeding strategies in Egypt. | ||
| Keywords | ||
| ISSR; SCoT; inter- intra variations; Olea europaea; Egypt | ||
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